hOGG1基因多态性与喉癌易感性的相关性分析  

Analysis of association of human 8-oxoguanine giycosylase 1 polymorphism with the susceptibility of laryngeal cancer

作　　者：李大鹏[1] 黄辉[1] 柴伟[1] 何苗[1] 邓洁[2] 张红蕾[3] 郭睿[2] 

机构地区：[1]安徽省亳州市人民医院耳鼻咽喉头颈外科,236800 [2]空军总医院耳鼻咽喉头颈外科,北京100142 [3]空军航空医学研究所附属医院耳鼻咽喉科,北京100089

出　　处：《中华耳鼻咽喉头颈外科杂志》2016年第9期681-684,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：中国共产党中央军事委员会后勤保障部十二五重大课题项目（AKJ11J003）

摘　　要：目的探讨人8－羟基鸟嘌呤糖苷酶1（human 8－oxoguanine giycosylase 1, hOGG1）基因多态性与喉癌易感性的相关性。方法采用对照研究，选择2010年1月至2014年12月期间在空军总医院及安徽省亳州市人民医院就诊的喉癌患者86例作为病例组，同期选择门诊体检的健康人86名作为对照组，两组均利用聚合酶链反应扩增及基因的测序分型技术行hOGG1基因多态性分析，分析其在两组间的差异。结果病例组携带hOGG1杂合型（Ser/Cys）及突变型（Cys/Cys）的比例均高于对照组，差异有统计学意义（P〈0.05），携带hOGG1杂合型（Ser/Cys）基因型的个体较野生型（Ser/Ser）基因型个体患喉癌的风险升高至2.97倍，而携带hOGG1突变型（Cys/Cys）基因型个体较携带野生型个体患喉癌的风险升高至8.09倍，按吸烟与否进行分层研究，发现吸烟人员中杂合型和突变型比例更高，差异有统计学意义（P〈0.05）。结论个体的hOGG1基因型为杂合型（Ser/Cys）或突变型（Cys/Cys）会增加罹患喉癌的风险，且与吸烟具有交互作用，其作为分子标志物对预测喉癌的发生具有一定的价值。ObjectiveTo study the association between human 8-oxoguanine giycosylase 1 （hOGG1） gene polymorphisms and the risk of laryngeal carcinoma. MethodsA total of 86 patients with laryngeal carcinoma treated in The Air Force General Hospital and the Bozhou People′s Hospital of Anhui Province from Jan 2010 to Dec 2014 were included in experimental group, and 86 healthy adults were selected as control group. The hOGG1 gene polymorphisms of subjects in two group were detected with by polymerase chain reaction amplification and gene sequencing technology, the differences in hOGG1 gene polymorphisms between two groups were analyzed. ResultsThe frequencies of hOGG1 gene heterozygous type （Ser/Cys） and mutant type （Cys/Cys） in experimental group were higher than those in control group（P〈0.05）. Compared to individuals with hOGG1 gene Ser/Ser, individuals with hOGG1 gene Ser/Cys had a 2.97-fold increased risk of laryngeal carcinoma, and individuals with hOGG1 gene Cys/Cys had a 8.09-fold increased risk of laryngeal carcinoma. Stratified by smoking or not, it was found that the proportion of heterozygous type or mutant type in smokers was higher significantly than non-smokers （P〈0.05）. ConclusionIndividuals with hOGG1 gene heterozygous type （Ser/Cys） or mutant type （Cys/Cys） have a higher risk of laryngeal carcinoma, with interaction between these gene types and smoking, and the gene types could be used as predictors for the occurrence of laryngeal carcinoma.

关 键 词：喉肿瘤 多态现象 遗传 疾病易感性 8-羟基鸟嘌呤糖苷酶1基因 

分 类 号：R739.65[医药卫生—肿瘤]