血管紧张素转化酶抑制肽LAP改善血压及心室重构的研究  

The effects of angiotensin-converting enzyme inhibitory peptide LAP to blood pressure and ventricular remodeling

作　　者：黄俊玲[1] 罗明[1] 谢南姿[1] 沈艺[1] 罗帮镇[1] 王海峰[1] 刘阳[1] 

机构地区：[1]同济大学附属同济医院老年科,上海200065

出　　处：《国际心血管病杂志》2016年第5期295-299,共5页International Journal of Cardiovascular Disease

基　　金：上海市自然科学基金(10ZR1433200);同济大学附属同济医院院级课题(TJ1505)

摘　　要：目的:探讨血管紧张素转化酶抑制肽LAP的长期降压效果及对心室重构的干预作用。方法:选取10周龄雄性自发性高血压大鼠(spontaneously hypertensive rat,SHR)为动物模型,随机分为空白对照组、依那普利组(Ena组)和LAP组。干预时间为8周,干预期间监测SHR的血压变化,干预结束后对SHR的心脏形态、心脏功能、心肌肥厚程度及心肌纤维化指标进行检测。结果:(1)干预2周后LAP组血压明显低于空白对照组(P<0.05),降压效果持续至干预结束;LAP组与Ena组比较血压无显著性差异。(2)干预8周后,与空白对照组相比,LAP组及Ena组室间隔厚度(IVST)、左室舒张末期内径(LVDd)、左室收缩末期内径(LVSd)、左室后壁收缩末期厚度(LVPWTs)、左室后壁舒张末期厚度(LVPWTd)明显下降(P<0.05),左室短轴缩短率(FS)、射血分数(EF)、心输出量(CO)明显升高(P<0.05);心室肌肥厚指数(VMI)明显下降(P<0.05);而LAP组与Ena组比较,上述各指标无显著性差异。(3)干预8周后,HE染色下,空白对照组心肌纤维结构明显紊乱,LAP组及Ena组较空白对照组心肌纤维明显纤细,纤维结构略有紊乱;Masson染色下,与空白对照组相比,LAP组及Ena组心肌胶原容积分数(CVF)和血管周围胶原面积(PVCA)明显下降(P<0.05),LAP组及Ena组间比较无显著性差异。结论:LAP对SHR可长期有效降压,能改善SHR的心脏功能,逆转左心室重构。Objective： To study the protective effects of angiotensiwconverting enzyme （ACE） inhibitory peptide LAP on blood pressure and ventricular remodeling. Methods： Ten-week-old male spontaneously hypertensive rats （SHR） were divided into three groups： blank control group, enalapril （Ena） group and ACE-inhibitory peptide LAP-treated （LAP） group. Each group was treated for 8 weeks. The alterations of blood pressure （BID）, cardiac morphology, cardiac function, cardiac hypertrophy and morphological changes of myocardial fibers were investigated. Results： （1） After 2 weeks intervention, the BP of LAP group was significantly lower than blank control group （P〈0. 05）, the effect lasted till the end of the study, the difference between LAP group and Ena group was not significant. （2） After 8 weeks intervention, as compared with blank control group, the IVST, LVDd,LVSd, LVPWTs, LVPWTd and VMI of LAP group and Ena group were significantly lower （P〈0. 05）, while FS, EF and CO were significantly higher （P〈0. 05）. But there was no significant differences between LAP group and Ena group. （3） After 8 weeks intervention, by HE staining, the structures of myocardial fibers in blank control group were obviously disordered. In LAP group and Ena group, myocardial fibers were significantly thinner than those in blank control group and their structures were slightly disordered. By Masson staining, the CVF and PVCA in LAP group and Ena group were significantly lower than those in blank control group （P〈0. 05）, but there was no significant difference between two groups. Conclusion： LAP has a long-term beneficial effect on BP of SHR. It can improve the SHRIs heart function and reverse left ventricular remodeling.

关 键 词：血管紧张素转化酶抑制肽 高血压 心室重构 

分 类 号：R544.1[医药卫生—心血管疾病]