SiO_2@NiO固相萃取结合超高效液相色谱—质谱法监测鸡蛋中阿苯达唑兽药及其代谢产物  

Monitoring albendazole and its metabolites in eggs by SiO_2@NiO solid-phase extraction coupled with ultra performance liquid chromatography-mass spectrometry

作　　者：余琼卫[1] 孙欢 刘平[1,2] 冯钰锜[1] YU Qiong-Wei SUN Huan LIU Ping FENG Yu-Qi(Key Laboratory of Analytical Chemistry for Biology and Medicine （Ministry of Education）, Department of Chemistry, Wuhan University, Wuhan 430072, China Pharmaceutical College Guangxi Medical University, Nanning 530021, China)

机构地区：[1]生物医学分析化学教育部重点实验室武汉大学化学与分子科学学院,武汉430072 [2]广西医科大学药学院,南宁530021

出　　处：《食品安全质量检测学报》2016年第8期3029-3033,共5页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31671929;21635006;31670373)~~

摘　　要：目的建立一种鸡蛋中阿苯达唑(albendazole,ABZ)兽药及其代谢产物阿苯达唑亚砜(albendazole sulfoxide,ABZ-SO)和阿苯达唑砜(albendazole sulfone,ABZ-SO_2)的新监测方法。方法将目前已知的苯并咪唑类药物标准样品直接进质谱分析,考察其二级碎裂质谱行为,找出其共同的特征子离子。鸡蛋样品经乙腈提取浓缩后,经5 mL 20 mmol/L磷酸盐缓冲液(p H 7.0)溶解,再经氧化镍涂层硅胶(SiO_2@Ni O)萃取柱进行固相萃取,最后采用m/z 159特征子离子进行母离子扫描监测阿苯达唑药物及其代谢产物。结果采用本方法监测到喂食阿苯达唑药物的母鸡产的鸡蛋中的阿苯达唑残留及其代谢产物,ABZ,ABZ-SO和ABZ-SO_2的检出限分别为2.2、5和3 ng/g。结论该方法快速、准确,适合食品中阿苯达唑及其代谢产物的分析监测。Objective To establish a novel method of monitoring albendazole（ABZ） and its metabolites（albendazole sulfoxide and albendazole sulfone, ABZ-SO and ABZ-SO） in eggs. Methods The fragmentation behaviour of benzimidazoles were investigated by the direct injection of benzimidazole standards for mass spectrometry（MS） analysis, and the common product ions were determined. Egg samples were extracted with acetonitrile and then concentrated, redissolved with 5.0 m L 20 mmol/L phosphate buffer（p H 7.0）. And then the albendazole and its metabolites in the extracts were processed by nickel oxide deposited on silica（SiO2@Ni O） solid-phase extraction. The albendazole and its metabolites which generated characteristic product ion m/z 159 were used for precursor ion scan in MS analysis. Results Using the established method, albendazole and its metabolites were detected in egg from the hens raised with albendazol. The limits of detection of ABZ, ABZ-SO and ABZ-SO2 were 2.2, 5 and 3 ng/g, respectively. Conclusion The proposed method is fast, accurate, which is suitable for monitoring albendazole and its metabolites residues in food samples.

关 键 词：阿苯达唑 母离子扫描 氧化镍 固相萃取 

分 类 号：O657.63[理学—分析化学] TS253.7[理学—化学]