常温下对骨肉瘤真空干燥灭活的探讨  

Exploration Study of the osteosarcoma devitalization technique by vacuum dehydration at room temperature

作　　者：陈飞[1] 朱夏[1] 吴朝阳[1] 沈荣凯[1] 林建华[1] Fei CHEN Xia ZHU Chaoyang WU Rongkai SHEN Jianhua LIN(Department of Orthopedic Oncology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Chin)

机构地区：[1]福建医科大学附属第一医院骨肿瘤科,福州市350005

出　　处：《中国肿瘤临床》2016年第17期741-746,共6页Chinese Journal of Clinical Oncology

基　　金：福建医科大学苗圃基金(编号:2010MP007);福建省临床重点专科项目(编号:闽卫科教[2012]149号)资助~~

摘　　要：目的:分析常温下对骨肉瘤组织进行真空干燥灭活的可行性。方法:体内实验对VX2肿瘤块进行真空干燥处理及冰水复水后,行体内种植,确定灭活处理的安全时间。体外实验对骨肉瘤组织进行真空干燥灭活,测定脱水率,观察肿瘤组织形态学改变,检测肿瘤组织ATP酶活性;检测兔骨、肌腱进行真空灭活处理后生物力学强度的变化。结果:常温下VX2肿瘤真空干燥灭活的安全时间为60 min;骨肉瘤组织接受真空干燥灭活后,明显皱缩,内部呈多孔状,脱水率达93.98%,镜下见细胞明显变小,结构破坏,透射电镜下见肿瘤细胞膜破坏,细胞器崩解,染色体分解;肿瘤组织的ATP酶活性明显较对照组降低;骨、肌腱的生物力学强度并未发生明显下降。结论:室温下真空干燥处理60 min,对骨、肌腱力学强度无明显影响,却可以使骨及软组织肿瘤内肿瘤细胞完全灭活。Objective： To demonstrate the feasibility of the osteosarcoma devitalization method by vacuum dehydration at room temperature. Methods： For the in vivo study, the VX2 tumor mass was treated by vacuum dehydration, rehydrated in ice water, and implanted in the rabbit to determine the safety time to deactivate the tumor. For the in vitro study, the osteosarcoma mass was devitalized by vacuum dehydration, and the dehydration rate and ATPase activity were determined. Histopathological changes in the tumor were also observed. The change in the biomechanical strength of rabbit bone and tendon after vacuum devitalization treatment was detected. Results： At room temperature, the safety time to deactivate the VX2 tumor was 60 rain, and the dehydration rate was 93.8% at this time point. After vacuum dehydration, the tumor mass evidently shrunk, presenting a porous structure. The osteosarcoma cell became small, and cell structure damage was observed under light microscope. Disrupted cell membrane and organelles were seen un- der transmission electron microscope as well as broken down chromosomes. The activity of ATPase was evidently lower than in the control group. The strength of bone and tendon did not decrease significantly after vacuum dehydration. Conclusion： Treatment by vacuum dehydration at room temperature for 60 rain does not result in differences of the bone and tendon strength. However, it can inactivate both soft tissue and bone tumor mass completely.

关 键 词：骨肉瘤 真空干燥 灭活 常温 

分 类 号：R738.1[医药卫生—肿瘤]