牙体充填材料表面粗糙度对常见口腔链球菌黏附力的影响  

Influence of surface roughness on oral streptococcal adhesion forces to dental filling materials

作　　者：郑赛男[1] 蒋丽[1] 张雷[1] 郝丽英[1] 叶露[1] 李伟[1] Zheng Sainan Jiang Li Zhang Lei Hao Liying Ye Lu Li Wei.(State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China)

机构地区：[1]口腔疾病研究国家重点实验室华西口腔医院(四川大学),成都610041

出　　处：《华西口腔医学杂志》2016年第5期448-453,共6页West China Journal of Stomatology

基　　金：国家自然科学基金(31200720);教育部博士学科点新教师基金(20120181120009);四川省科技计划项目(2016FZ0069)~~

摘　　要：目的采用原子力显微镜(AFM)检测常见口腔链球菌属与不同表面粗糙度的光固化复合树脂及玻璃离子水门汀(GIC)之间的黏附力。方法将光固化复合树脂和GIC样本表面梯度抛光,根据最终表面粗糙度不同分为300、200、100和10 nm组,使用AFM观察其表面形貌。采用先锋菌(血链球菌、缓症链球菌)和致龋菌(变异链球菌、表兄链球菌)制作细菌改性探针,通过AFM获得力—距曲线测量细菌与树脂和GIC样本表面的黏附力。对材料表面粗糙度测量值进行方差分析,细菌黏附力进行Kruskal-Wallis非参数检验,同时采用Dunn’s进行组间两两比较,并对表面粗糙度与细菌黏附力进行相关性分析。结果随材料表面粗糙度增加,细菌的黏附力增大,4种细菌的黏附力均在300 nm的材料表面达到最大值;在10和300 nm组的GIC表面,变异链球菌的黏附力由0.578 n N增加到2.876 n N。4种细菌在树脂表面的黏附力略大于GIC,先锋菌的黏附力略大于致龋菌,组间差异均在200和300 nm组时较明显。结论材料表面粗糙度对细菌黏附力的影响较大,二者有明显的相关性;GIC对细菌的黏附性较复合树脂低;材料表面粗糙度对致龋菌的影响小于先锋菌。Objective This study is to determine the common oral streptococcal adhesion forces by using composite resin and glass ionomer cement （GIC） with different degrees of surface roughness via atomic force microscopy （AFM） analysis. The influence of surface roughness on bacterial adhesion force is also discussed. Methods Polishing and grinding were applied to obtain 300, 200, 100, and 10 nm surfaces of light-cured composite resin and GIC samples. Surface topography was assessed by AFM analysis. Initial colonizers （Streptococcus sanguinis and Streptococcus mitis） and cariogenic bacterial strains （Strep- tococcus mutans and Streptococcus sobrinus） were used to obtain bacteria-modified AFM probes. The force-distance curves were also measured by AFM analysis to determine the adhesion forces of bacteria on the surfaces of the composite resin and GIC. Material surface roughness was analyzed using ANOVA, and adhesion forces were subjected to nonparametric analysis （Kruskal-Wallis test）. Comparison among groups was performed by Dunn＇s test. Material surface roughness and bacterial adhesion forces were subjected to correlation analysis. Results Bacterial adhesion forces increased with increasing material roughness. The adhesion forces of the four bacterial species reached the maximum on the material surface of 300 nm. The adhesion force of Streptococcus mutans increased from 0.578 nN to 2.876 nN on GIC surfaces with 10 and 300 nm roughness. The adhesion forces of the four species on the surface of the composite resin were stronger than that of GIC. The initial colonizers exhibited stronger adhesion forces to different materials than the cariogenic strains. Intergroup differences were evident on the 200 and 300 nm material surfaces. Conclusion The surface roughness of the material significantly affected the bacterial adhesion forces, and a sig- nificant linear correlation existed between both factors. The bacterial adhesion forces of the GIC were lower than that of the composite resin. Furthermore, surface

关 键 词：原子力显微镜 黏附力 充填材料 表面粗糙度 口腔链球菌属 

分 类 号：R781.1[医药卫生—口腔医学]