TPD52在胶质瘤中的表达水平及临床意义  

Expression level of TPD52 in gliomas and its clinical significance

作　　者：高兵兵[1] 秦浩[1] 秦家振[2] 孙移坤 张昊驹 刘晨[2] 侯江雷 黄佛宝 罗丹[2] 戴宜武[1] Gao Bingbing Qin Hao Qin Jiazhen et al(Affiliated Bayi Brain Hospital of General Hospital of Beijing Military Command, Anhui Medical University, Beifing 100000 Affiliated Bayi Brain Hospital of General Hospital of Beijing Military Command, Beijing 100000)

机构地区：[1]安徽医科大学北京军区总医院附属八一脑科医院,北京100000 [2]北京军区总医院附属八一医院脑科医院,北京100000

出　　处：《安徽医科大学学报》2016年第10期1521-1524,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81271391)

摘　　要：目的探究TPD52在胶质瘤及正常脑组织中的表达及其临床意义。方法收集46例胶质瘤患者病例标本及瘤旁正常脑组织,应用荧光定量PCR、Western blot、免疫组织化学染色方法检测TPD52 mRNA、蛋白的表达量,并分析临床指标与TPD52表达量之间的关系。结果荧光定量PCR及Western blot结果具有一致性,均提示TPD52的表达量随胶质瘤病理分级升高,Ⅲ、Ⅳ级胶质瘤TPD52表达量明显高于正常脑组织、Ⅰ、Ⅱ级胶质瘤,差异有统计学意义(P<0.05)。免疫组织化学染色显示TPD52蛋白定位在胶质瘤细胞质中,67.4%病理标本高表达TPD52(或)。结论TPD52 mRNA与蛋白在胶质瘤细胞中高表达,其蛋白表达量与胶质瘤病理分级呈正相关性,与生存期呈负相关性,其可能是胶质瘤临床治疗的一个潜在基因靶点。Objective To explore the expression of tumor protein D52 （TPD52） in human glioma and normal brain tisse and its clinical significance. Methods Collecting specimens of 46 cases of gliomas and normal brain tissue ad- jacent to tumours. The expression of TPD52 was investigated by real-time quantitative PCR, Western blot and immu- nohistochemistry in gliomas. The relationships betwen TPD52 espression and clinical factors were analyzed. Results The results of real-time quantitative PCR and Western blot showed that the expression of TPD52 mRNA and pro- tein significantly increased in glioma tissues as compared with those in normal brain tissues. Immunohistochemistry displayed that 67.4% glioma tissues expressed higher TPD52 protein. Conclusion The expressions of TPD52 mR- NA and protein are significantly higher in glioma than normal brain tisse, and the expression of TPD52 protein is positively associated with glioma WHO classification and negatively associated with patients lifetime. It may be a potential target genes to treat gliomas.

关 键 词：胶质瘤 TPD52 病理分级 生存期 

分 类 号：R730.26[医药卫生—肿瘤]