亚慢性暴露壬基酚诱发大鼠非酒精性脂肪肝的研究  

Study of non-alcoholic fatty liver disease in rats induced by nonylphenol subchronic exposure

作　　者：杨雪松[1] 俞捷[1] 杨雪峰[2] 罗娅[1] 杨静[1] 李克彬[1] 许洁[1] 

机构地区：[1]遵义医学院公共卫生学院,563000 [2]遵义医学院附属医院胃肠外科,遵义563099

出　　处：《重庆医学》2016年第28期3900-3903,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(201360439;81560527);贵州省教育厅青年基金资助项目(黔教合KY字[2013]198);贵州省卫生厅基金资助项目(gzwkj20131127);遵义医学院招标基金资助项目(2013F-68);遵义医学院研究生社会实践基金资助项目(ysh-2015-001);贵州省科技厅基金资助项目(黔科合LH字[2014]7543;黔科合J字[2014]2177号;[2014]2185号)

摘　　要：目的探讨连续90d灌胃壬基酚(NP)能否诱发非酒精性脂肪肝(NAFLD)。方法 48只雄性SD大鼠分成4组,即对照组,低、中、高剂量组,NP剂量分别为0、20、60、180mg·kg-1·d-1,每组平均分为常规饮食、高脂饮食两个亚组。空腹灌胃NP 90d,期间每周称量1次体质量,第60天行肝脏彩超观察。第90天全自动生化分析仪检测肝功、血脂,高效液相色谱仪检测肝脏中NP的浓度。结果各剂量常规饮食组与对照组比较,低、中、高剂量组三酰甘油(TG)降低(P>0.05),天门冬酸氨基转移酶(AST)升高(P<0.05),低、高剂量组丙氨酸氨基转移酶(ALT)降低(P<0.05),低密度脂蛋白(LDL)减低(P<0.05),对照组及低、中剂量组未出现脂肪肝,高剂量组1/2的动物出现脂肪肝。各剂量高脂饮食组与对照比较,低、中、高剂量组、TG升高(P<0.05),ALT降低(P<0.05),AST升高(P<0.05),HDL升高(P<0.05),高剂量组总胆固醇(TC)升高(P<0.05),高脂饮食组被选部分动物中,低、中剂量组1/2的动物出现了脂肪肝,高剂量组则全部出现脂肪肝病变。与对照组比较,低、中、高剂量组大鼠肝脏NP浓度升高(P<0.05),肝脏NP浓度与染毒剂量的存在秩相关rs常规饮食=0.942,rs高脂饮食=0.481(P<0.05)。结论亚慢性染暴露NP是导致NAFLD的危险因素之一,增加了NAFLD的发病风险。Objective To explore whether expose to nonyphenol（NP）can induce non-alcoholic fatty liver disease（NAFLD）in rats, subchronically. Methods Totally 48 male SD rats were randomly divided into 4 groups,namely,control,low,middle, and high dose group,the doses were 0,20,60,180 mg · kg-1·d-1 , each group was divided into normal diet and night fat diet equally. The rats were intragastric administration of NP 90 d,weights of rats were examined once a week,on the 60th day ,livers were detected by colored type-B ultrasonic. On the 90th day,liver function and blood fat were tested by automatic biochemistry analyzer, content of liver＇s nonyphenol examed by high performance liquid chromatograph. Results In normal diet,compare with control group,low, middle and high dose group of glycerol triglyeeride（TG） was decreased（P〈0.05） ,aspartate amino shift enzyme（AST） increased in middle and high groups（P〈0.05） ,alanine transfer enzyme（ALT）decreased（F= 12. 973 ,P〈0.05）in low and high group,low density lipoprotein（LDL）decreased（P〈0.05）. In normal diet, there was no occurrence of NAFLD in control, low and middle groups, but a half of animals were suffered from NAFLD. In the high fat diet,compared with control group, ALT was decreased in low, mid- dle and high dose（P〈0.05） ,AST was rose（P〈0.05） and TG was increased（F=2. 979, P〈0.05） in high dose group, the total eholesterol（TC） was increased（P〈0.05） ,in low dose group and middle dose group, HDL was increased（P〈0.05）. In some selected animals of the high fat diet group, half of the animals in the low and middle dose groups occurred NAFLD, while in high dose group all occurred NAFLD. Compared with the control group, concentration of NP in rat liver were increased in treatment gounp （P〈0.05）, NP concentration and the exposure,dose exit rank correlation coefficient（rs l diet = 0. 942 ,rsgugh dose =0. 481 P〈0.05）. Conclusion Exposure to NP is one of the risk factors which leading to NAFLD, subchr

关 键 词：壬基酚 非酒精性脂肪肝 常规饮食 高脂高糖饮食 

分 类 号：R575.5[医药卫生—消化系统]