川芎嗪联合丹参酮ⅡA对人肺巨细胞癌PG-BE1、PG-LH7细胞增殖、黏附及侵袭的影响  

Effect of Tetramethylpyrazine Combined with Tanshinone ⅡA on the Proliferation,Adhesion and Invasion of Human Lung Giant Cell Carcinoma Cell Lines PG-BE1 and PG-LH7

作　　者：韩海英[1] 张培彤[1] 王耀焓[1] 郭秀伟[1] 

机构地区：[1]中国中医科学院广安门医院,北京100532

出　　处：《中国肿瘤》2016年第9期728-733,共6页China Cancer

基　　金：国家自然科学基金(81173450;81302963)

摘　　要：[目的]探讨不同浓度川芎嗪(TMP)联合丹参酮ⅡA(TanⅡA)对人肺巨细胞癌细胞高转移亚系PG-BE1(PB)、低转移亚系PG-LH7(PL)增殖、黏附及侵袭的影响。[方法]不同浓度的TMP+TanⅡA作用于PB、PL细胞24h、48h、72h后,CCK8检测不同给药组对PB与PL细胞的增殖抑制作用,采用人造基底膜实验与Transwell小室侵袭实验检测细胞黏附及侵袭能力的差异。[结果]TMP+TanⅡA对PB、PL细胞增殖的影响随着浓度增加抑制率增高;随着作用时间的延长,对PB细胞抑制作用增强,对PL细胞抑制作用减弱。TMP+TanⅡA对PB、PL细胞黏附的抑制作用随着药物浓度的增加而增强;在侵袭实验中,TMP+TanⅡA对PB细胞侵袭的抑制率高于PL细胞,其抑制作用也随着药物浓度的增加而增强。[结论]TMP+TanⅡA对人肺巨细胞癌细胞高转移亚系PB、低转移亚系PL具有明显的增殖、黏附及侵袭抑制作用。细胞种系不同,药物的作用时间与浓度对细胞的抑制率也不同。[Purpose]To observe effect of tetramethylpyrazine combined with Tanshinone Ⅱ A （Tan Ⅱ A） on the proliferation, adhesion and invasion of human lung giant cell carcinoma cell lines PG- BE1 （PB） and PG-LHT（PG）. [Methods] Different concentration gradient TMP＋TanⅡ A was cultivat- ed PG-BE1 and PG-LH7 within different time （24h,48h,72h）. The effect of anti-proliferation was observed by CCK8, and the abilities of invasion and adhesion were detected by Matrigel invasion assay and cell adhesion assay. [ Results ] TMP＋Tan ⅡA has certain inhibitory effect on the prolifer- ation of PB cells and PL cells. The influence of anti-proliferation increased with the drug concen- tration raise. With the extension of time,the inhibitory effect of PB cells was increased,but the in- hibitory effect of PL cells was decreased. The influence of TMP＋TanⅡ A on adhesion and invasion ability of PB and PL cells was increased with the concentration raise. In transwell test,different concentration TMP＋Tan ⅡA has stronger inhibiting effect to PB cells than PL cells, [Conclusion] Different concentration gradient TMP＋Tan Ⅱ A can inhibit the proliferation,adhesion and invasion of PB and PL cells. Because cells type, the action time＇ and concentration of drug are different, so the inhibitory effect on PB and PL is different.

关 键 词：川芎嗪 丹参酮ⅡA 肺癌 增殖 黏附 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]