miR-539对乳腺癌细胞增殖与凋亡的调控作用研究  

Regulated Role of miR-539 on Proliferation and Apoptosis in Breast Cancer Cells

作　　者：佘青[1] 张锦[1] 李有怀[1] 程永刚[1] 李昭琦[1] 杨文强[1] 王磊[1] 

机构地区：[1]宝鸡市中心医院乳腺科,陕西宝鸡721008

出　　处：《解放军医药杂志》2016年第9期55-58,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：陕西省科学技术研究发展计划(2010k15-06-04)

摘　　要：目的研究miR-539对乳腺癌细胞增殖和凋亡的调控作用。方法采用荧光定量PCR检测乳腺癌细胞(MCF-7、MDA-MB-453、ZR-75-30)和正常乳腺上皮细胞(MCF-10A)中miR-539表达的差异;通过转染miR-539-mimics在MCF-7细胞中上调miR-539的表达,并通过MTT和TUNEL实验检测miR-539对乳腺癌细胞增殖和凋亡的影响。结果与正常乳腺上皮细胞相比,乳腺癌细胞中miR-539的表达显著降低(P<0.01)。与空白对照组和阴性对照组相比,过表达miR-539能显著降低过表达组乳腺癌MCF-7细胞的增殖能力并增加其对地塞米松诱导凋亡的敏感性(P<0.01)。结论 miR-539能够抑制乳腺癌细胞增殖并促进其凋亡,有望作为乳腺癌基因治疗的靶点。Objective To investigate regulation function of microRNA-539 （miR-539） on proliferation and ap-optosis of breast cancer cells. Methods Expressions of miR-539 in breast cancer cells （MCF-7, MDA-MB-453 and ZR-75-30） and normal breast epithelial cells （MCF-10A） were detected by fluorescent quantitation polymerase chain reaction （ PCR） . miR-539 expression was up-regulated by transfecting miR-539-mimics into MCF-7 cells, and then effects of miR-539 on proliferation and apoptosis of breast cancer cells were detected by MTT assay and TUNEL experiment. Re-sults miR-539 expression in breast cancer cells was significantly decreased compared with that in normal breast epitheli-al cells （P〈0. 01）. Proliferation ability of MCF-7 breast cancer cells was significantly decreased, and its sensitivity to apoptosis induced by Dexamethasone was increased in over-expressed miR-539 group compared with those in control and negative groups （P〈0. 01）. Conclusion MiR-539 can inhibit proliferation and promote its apoptosis of breast cancer cells, which may be used as a target for gene therapy of breast cancer.

关 键 词：微RNA-539 乳腺肿瘤 细胞增殖 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]