急性STEMI患者血同型半胱氨酸与Kv1.3通道、肌钙蛋白关系的研究  

Study on relationship between plasma homocysteine,Kv1.3 channel and troponin in patients with acute ST-segment elevation myocardial infarction

作　　者：王玉岭[1,2] 冯建宇[3] 曾祥飞[2] 杨绍兵[2] 严宁[2] 贾绍斌[4] 

机构地区：[1]洛川县妇幼保健院,延安727400 [2]宁夏医科大学 [3]第四军医大学西京医院心脏外科 [4]宁夏医科大学总医院心脏中心

出　　处：《天津医药》2016年第10期1263-1267,共5页Tianjin Medical Journal

基　　金：宁夏医科大学优势学科群建设科研项目(xy2014016)

摘　　要：目的探讨急性ST段抬高型心肌梗死(STEMI)患者血浆同型半胱氨酸(Hcy)水平与淋巴细胞电压门控性钾通道(Kv1.3通道)、Kv1.3通道与心肌肌钙蛋白I(c Tn I)水平的相关性。方法 80例STEMI患者根据血浆Hcy水平分为STEMI合并高同型半胱氨酸血症(Hhcy)组(STEMI+Hhcy组,Hcy>15μmol/L,n=41)和对照组(STEMI组,Hcy≤15μmol/L,n=39)。抽取血标本后全自动生化仪测定Hcy、c Tn I及血脂等指标,密度梯度离心法提取淋巴细胞,实时荧光定量PCR检测Kv1.3 m RNA表达,Western blot检测Kv1.3蛋白表达。结果 STEMI+Hhcy组c Tn I水平高于STEMI组(μg/L:22.997±5.880 vs.12.881±6.343;P<0.01)。多元线性回归分析显示年龄、性别、高血压病、糖尿病、吸烟史、家族史、三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)对Hcy无明显影响(P>0.05)。STEMI+Hhcy组Kv1.3通道m RNA和蛋白相对表达量(1.35±0.14和0.85±0.12)较STEMI组升高(1.00±0.07和0.64±0.05,P<0.05)。Hcy水平与Kv1.3通道m RNA和蛋白表达水平呈正相关(r分别为0.299、0.542,P<0.05);Kv1.3通道蛋白表达水平与c Tn I水平呈正相关(r=0.644,P<0.05)。结论 Hcy可能对淋巴细胞Kv1.3通道表达起着一定的作用,导致血浆c Tn I水平升高。Objective To investigate the relationship between plasma homocysteine(Hcy),Kv1.3 channel and cardiac troponinl(cTnI) in patients with acute ST- segment elevation myocardial infarction(STEMI).Methods According to the level of Hcy,80 STEMI patients were divided into STEMI with Hhcy group(Hcy>15 μmol/L,n=41) and control group(STEMI group,Hcy≤15 μmol/L,n=39).The Hcy,blood lipid and cTnI were detected with automatic biochemistry analyzer,respectively.Peripheral lymphocytes were isolated by ficoll density gradient centrifugation.Real- time PCR was used to detect mRNA expression of Kv1.3,and Western blot assay was used to detect protein expression of Kv1.3.Results cTnI concentrations were obviously higher in STEMI with Hhcy group than those in STEMI group(μg/L:22.997 ±5.880 vs.12.881 ± 6.343;P< 0.01).Multiple linear regression analysis showed that age,gender,hypertension,diabetes mellitus,smoking,family history,total cholesterol(TC),triglyceride(TG),high density lipoprotein- cholesterol(HDL- C) and low density lipoprotein-cholesterol(LDL-C) had no obvious influence on Hcy(P>0.05).The relative expression levels of Kv1.3mRNA and protein were significantly higher in STEMI with Hhcy group(1.35±0.14,0.85±0.12) than those in STEMI group(1.00 ±0.07,0.64 ±0.05,P<0.05).Moreover,there was a positive relation between Hcy level and the mRNA and protein expression of Kv1.3 channel(r=0.299,r=0.542,P<0.05).There was a positive relation between protein expression levels of Kv1.3 channel and cTnI(r=0.644,P<0.05).Conclusion Our results support that Hcy could exacerbate the concentration of cTnI through playing an important role in the Kv1.3 mRNA and protein expression in lymphocytes.

关 键 词：心肌梗死 高同种半胱氨酸血症 Kv1.3钾通道 淋巴细胞 急性ST段抬高型心肌梗死 

分 类 号：R541.4[医药卫生—心血管疾病]