蓝莓联合益生菌对非酒精性脂肪性肝病模型大鼠PNPLA3和SREBP-1c表达的影响  

Effect of blueberry and probiotics on expression of PNPLA3 and SREBP-1c in rats with nonalcoholic fatty liver disease

作　　者：祝娟娟[1] 周明玉[1] 程明亮[1] 

机构地区：[1]贵州医科大学附属医院感染科,贵阳550004

出　　处：《临床肝胆病杂志》2016年第9期1778-1784,共7页Journal of Clinical Hepatology

基　　金：贵州省科技厅社会攻关资助项目(黔科合SY[2010]3017号);黔东南苗族侗族自治州林业局科研基金资助项目(ZLYJZFCG-2012-7)

摘　　要：目的观察含patatin样磷脂酶域3(PNPLA3)和胆固醇调节元件结合蛋白(SREBP)1c在蓝莓联合益生菌对非酒精性脂肪性肝病(NAFLD)模型大鼠干预实验中的表达,并探讨其在NAFLD中的作用机制。方法清洁级SD大鼠36只分为正常组(NG)、模型组(MG)、自然恢复组(SRG)、蓝莓组(BG)、益生菌组(PG)和蓝莓联合益生菌组(BPG)。实验结束后,制作组织切片,观察血清学、组织学形态变化,检测PNPLA3和SREBP-1c基因及蛋白表达情况。多组间比较用单因素方差分析,方差齐时用SNK-q检验,方差不齐时用Tamhane's-T2检验。结果 6组间在肝脏指数、ALT、TC、TG、LDL和HDL上差异均有统计学意义(F值分别为384.908、188.554、75.523、94.667、95.235、132.586,P值均<0.01)。6组间NAFLD活动度积分差异有统计学意义(F=71.896,P<0.01),其中BPG组较MG、SRG、BG、PG组下降最为显著(P值均<0.01)。6组肝组织表达PNPLA3和SREBP-1c阳性率差异均有统计学意义(F值分别为175.527、110.504,P值均<0.01),其中BPG组较MG、SRG、BG、PG组下降最为显著(P值均<0.01)。PNPLA3和SREBP-1c的mRNA和蛋白表达各组间差异有统计学意义(F值分别为375.822、410.379、288.940、116.054,P值均<0.01)。其中BPG组的PNPLA3和SREBP-1C的mRNA和蛋白表达较MG、SRG、BG、PG组明显下降(P值均<0.01)。结论蓝莓联合益生菌能有效改善NAFLD的病理组织结构,减轻肝细胞脂肪变性,其机制可能是蓝莓与益生菌联合可减轻炎症因子诱发的炎症效应,下调SREBP-1c的表达,从而减弱PNPLA3基因转录,增强胆固醇代谢,减少脂质沉积,是NAFLD的一个辅助治疗方案。Objective To investigate the expression of patatin- like phospholipase domain- containing protein 3（ PNPLA3） and sterol regulatory element- binding protein- 1c（ SREBP- 1c） in the rat model of nonalcoholic fatty liver disease（ NAFLD） treated by blueberry combined with probiotics,as well as its mechanism of action in NAFLD. Methods A total of 36 clean Sprague- Dawley rats were divided into normal group（ NG）,model group（ MG）,spontaneous recovery group（ SRG）,blueberry group（ BG）,probiotics group（ PG）,and blueberry- probiotics group（ BPG）. Tissue sections were prepared after the experiment ended to observe serological and histological changes and measure the mRNA and protein expression of PNPLA3 and SREBP- 1c. A one- way analysis of variance was used for comparison between multiple groups; the SNK- q test was used for data with homogeneity of variance,and the Tamhane＇s- T2 test was used for data with heterogeneity of variance. Results The six groups showed significant differences in liver index,serum alanine aminotransferase,total cholesterol,triglyceride,low- density lipoprotein,and high- density lipoprotein（ F = 384. 908,188. 554,75. 523,94. 667,95. 235,and132. 586,all P〈0. 01）. The NAFLD activity score also showed a significant difference between the six groups（ F = 71. 896,P〈0. 01）,and BPG showed a significantly greater reduction in the NAFLD activity score than MG,SRG,BG,and PG（ all P〈0. 01）. The six groups showed significant differences in the positive rates of PNPLA3 and SREBP- 1c in liver tissue（ F = 175. 527 and 110. 504,both P〈0. 01）,and BPG showed a significantly greater reduction in the positive rates of PNPLA3 and SREBP- 1C than MG,SRG,BG,and PG（ all P〈0. 01）. The mRNA and protein expression of PNPLA3 and SREBP- 1c showed a significantly difference between the six groups.（ F =375. 822,410. 379,288. 940,116. 054,all P〈0. 01）. BPG showed significant reductions in the mRNA expression of PNPLA3 and SREBP- 1c compared with MG,SRG,BG,and

关 键 词：脂肪肝 含patatin样磷脂酶域3 胆固醇调节元件结合蛋白质1 大鼠 SPRAGUE-DAWLEY 

分 类 号：R575.5[医药卫生—消化系统]