锦带花纤孔菌液体培养过程中的抗氧化活性  

Antioxidant Activity of Inonotus weigelae During the Liquid Cultivation

作　　者：孟歌[1] 郑飞[1] 刘红霞[2] 司静[1] 崔宝凯[1] 

机构地区：[1]北京林业大学微生物研究所,北京100083 [2]北京林业大学林学院,北京100083

出　　处：《生物技术通报》2016年第9期140-148,共9页Biotechnology Bulletin

基　　金：中央高校基本科研业务费专项资金资助项目(BLX2014-36;JC2013-1);国家自然科学基金项目(31422001)

摘　　要：为更好地研究和开发药用真菌锦带花纤孔菌(Inonotus weigelae)的药理作用,以菌丝体生物量、p H、还原糖含量、漆酶活性、多酚含量、丙二醛(Malonaldehyde,MDA)含量、超氧化物歧化酶(Superoxide dismutase,SOD)活性、总抗氧化能力(Total antioxidant capacity,T-AOC)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力为检测目标,对锦带花纤孔菌在液体培养过程中的抗氧化活性进行了评价。结果显示,抗氧化活性与菌株生长状况、胞外酶活性及次级代谢产物分泌密切相关。菌丝体生物量增长呈"S"型,6-12 d迅速增长,第12天达到最大值,在此过程中还原糖含量、漆酶活性及多酚含量均出现高峰,且菌丝体生物量的变化趋势与漆酶活性和多酚含量基本一致,说明菌株的营养利用与生理代谢相互依存。SOD活性与T-AOC在培养过程中均呈上升趋势,说明菌株在氧化胁迫的压力下可启动自身的抗氧化系统以抵御外界的损伤。此外,锦带花纤孔菌对DPPH自由基的清除能力也较强,第2天时清除率为78.56%,第14天时仍能达到50%以上。结果证明药用真菌锦带花纤孔菌具有较强的抗氧化活性。In order to better study and develop the i^harmacological effect of the medicinal fungus Inonotus weigelae in the future, based on the tested indicators of mycelial biomass, pH, reducing sugar content, laccase activity, polyphenol content, MDA （Malonaldehyde） content, SOD （ Superoxide dismutase ） activity, T-AOC （ Total antioxidant capacity ）, and DPPI-I （ 1,1-diphenyl-2-picrylhydrazyl ） radicals scavenging activity, the antioxidant activity of I. weigelae was evaluated during the liquid cultivation. The results demonstrated that the strain＇ s antioxidant activity was closely correlated with its mycelial growth, extracellular enzyme activity, and secondary metabolite secretion. The variations of mycelial biomass presented S-like curve, which increased rapidly from 6 to 12 d, reaching its peak at the 12th day. During the liquid cultivation, the increased peaks on reducing sugar content, laccase activity, and polyphenol content were also observed. Variation trends among the mycelial biomass, laccase activity, and polyphenol content were approximately consistent, indicating that there existed a mutual dependence between the nutrition utilization of this fungal strain and its physiological metabolism. The SOD activity and T-AOC during the euhurd tended to increase, indicating that the strain activated its antioxidant system to resist the damage from external environment under the oxidative stress. Additionally, I. weigelae exhibited remarkable scavenging capacity against DPPH radicals, showing the scavenging efficiency was up to 78.56% on the 2nd day and remaining over 50% on the 14th day. The above results confirmed that the medicinal fungus I. weigelae possesses the superior antioxidant activity.

关 键 词：药用真菌 锦带花纤孔菌 菌丝体生物量 漆酶活性 抗氧化活性 

分 类 号：R285[医药卫生—中药学]