fat-1转基因牛血浆差异蛋白的研究  

Study of Plasma Differential Proteins in fat-1 Transgenic Cow

作　　者：刘新峰[1,2] 丁向彬[2] 王轶敏[2] 李新[2] 郭宏[2] 李光鹏[1] 

机构地区：[1]内蒙古大学国家转基因动物技术研究中心,呼和浩特010070 [2]天津农学院动物科学与动物医学学院,天津300384

出　　处：《生物技术通报》2016年第9期203-209,共7页Biotechnology Bulletin

基　　金：国家转基因生物新品种培育重大专项(2014ZX08007-002)

摘　　要：以fat-1转基因牛血浆蛋白为材料,利用2D-双向电泳、生物信息学及血脂生化检测技术对fat-1基因调控牛脂类代谢的潜在机制进行了研究。结果表明,8个相关差异蛋白(AHSG、KNG1、Serpin A3-1、ENSBTAG00000021729、Serpin A3-5、FAM208A、CTAGE5、LOC511240)被鉴定;经生物信息学预测,发现有63个蛋白与这8个差异蛋白存在互作关系,而且发现差异蛋白和互作蛋白富集了11个与脂类代谢密切相关的生物学通路,其中互作蛋白APOA1在11个通路中富集的频率最高;经血浆APOA1检测发现,转基因牛显著高于野生型牛;相关性分析发现APOA1与低密度脂蛋白胆固醇(LDL-C)高度负相关(r=-0.90),与高密度脂蛋白胆固醇/总胆固醇(HDL-C/TC)的比值存在显著正相关(r=0.69)。综合结果显示了fat-1基因可能通过介导APOA1的表达调控转基因牛的脂类代谢。Using the plasma proteins in fat-] transgenic cow as the research material, the potential mechanism of fat-1 regulating lipid metabolism were studied using two-dimensional electrophoresis, bioinformatics and plasma lipid biochemical detection technology. The result showed that 8 differential proteins （AHSG, KNG1, Serpin A3-1, ENSBTAG00000021729, Serpin A3-5, FAM208A, CTAGES, and LOCS11240 ） were identified. Bioinformatics predicted that there were 63 proteins interacting with these 8 differential proteins, and it was discovered that differential proteins and interacting proteins enriched in 11 biological pathways related to lipid metabolism, and the APOA1 of which enriched at the highest frequency in the 11 pathways. Accordingly, the significantly higher plasma level of APOA1 was detected infat- 1 transgenic cow than wild-type cow. Then, the plasma level of APOA1 was negatively related to the plasma level of low density lipoproten- cholesterol （ LDL-C, r=-0.90 ）, whereas positively related to the ratio of high density lipoproten-cholesterol/total cholesterol （ HDL-C/TC, r=0.69 ） . These results indicated the fat-1 gene regulated the lipid metabolism by mediating the expression of APOA1 in transgenic cow.

关 键 词：fat-1转基因牛 血浆蛋白 n-3 PUFAS 脂类代谢 

分 类 号：S823[农业科学—畜牧学]