油茶枝浸提液对茶薪菇菌丝酚类物代谢及抗氧化特性的影响  

Effects of Camellia oleifera Abel Extraction on Enhancing Mycelial Metabolism for Phenolic Compounds with Agrocybe chaxingu Huang and Antioxidant Function

作　　者：吴俐[1] 沈恒胜[1] 汤葆莎[1] 杨艺龙[1] 陈君琛[1] 

机构地区：[1]福建省农业科学院农业工程技术研究所,福州350003

出　　处：《中国食品学报》2016年第7期59-64,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省自然科学基金(2011J01100);福州市科技计划项目(2014-G-63)

摘　　要：以未添加油茶枝浸提液的培养基为对照,比较添加2%,4%,8%油茶枝浸提液的茶薪菇液体培养效果,探讨对菌丝生物量、胞内外总酚含量,以及菌丝体提取液和发酵液清除羟自由基、超氧阴离子和抗脂质过氧化等抗氧化活性的影响。结果表明,油茶枝浸提液作为营养添加底物,可明显促进菌丝生长及其对酚类物的代谢转化,且作用效果随添加水平而增强,以8%添加量为最佳。浸提液的营养底物具有明显的优化菌丝的体外抑制卵黄脂质过氧化作用,8%添加组较CK组提高89.4%;菌丝的体外羟自由基清除作用却依添加水平而降低,8%添加组较CK组降低25.7%。受基质浸提液对菌丝胞外代谢转化作用的影响,发酵液中残留总酚含量及其体外抗氧化特性随添加水平而提高,尤以对清除羟自由基能力的优化效果明显。研究证实,8%油茶枝浸提液为最佳添加水平。Fermentation effects of different supplementary levels（2%, 4%, 8%） of Camellia oleifera branches extracts（Co-BE） compared with the control（liquid medium without Co-BE） were investigated. The influence of biomass and total phenols in mycelia and culture broth, and their antioxidant activity including scavenging rate to hydroxyl radicals, superoxide anion and antiperoxidation of lipid were studied. The results show that mycelia growth and phenolic metabolic transformation were continuously promoted with the additional level of Co-BE as nutrition substrate. So 8% was the optimal concentration of Co-BE. Co-BE obviously optimized antiperoxidation of yolk lipid in vitro of mycelia that of8% group was higher 89.4% than CK; However, hydroxyl radical scavenging in vitro of mycelia was decreased with additional level, scavenging rate of 8% group was 25.7% lower than CK. Because of the effect on extracellular metabolic conversion of Co-BE, the remaining total phenols of culture broth and its antioxidant properties in vitro increased with additional levels, Co-BE had obvious effect on scavenging capacity to hydroxyl radicals. Research of antioxidant activity confirmed that 8% Co-BE was the optimal concentration.

关 键 词：油茶 茶薪菇 多酚 液体培养 抗氧化 

分 类 号：S646[农业科学—蔬菜学]