啤酒中玉米赤霉烯酮的高效液相色谱紫外检测法  

Determination of zearalenone in beer by high-performance liquid chromatography with UV detection

作　　者：张婷[1] 向仲朝[1] 

机构地区：[1]绵阳市疾病预防控制中心,四川绵阳621000

出　　处：《现代预防医学》2016年第19期3590-3592,共3页Modern Preventive Medicine

摘　　要：目的建立啤酒中玉米赤霉烯酮的高效液相色谱紫外检测法。方法啤酒中的玉米赤霉烯酮用乙酸乙酯提取,50℃水浴中氮气吹干,用乙腈溶解,过滤,以Zorbax SB-18为分离柱,用甲醇+0.020 mol/L乙酸铵(75+25)溶液为流动相,检测波长236nm。结果玉米赤霉烯酮在0μg/ml^10.0μg/ml范围内,线性关系良好(r=0.999 97);方法检出限5.0μg/kg。方法相对标准偏差(RSD)为2.3%~2.7%,加标回收率为86.0%~100.0%,平均回收率为93.6%。啤酒样品中玉米赤霉稀酮含量为51.6μg/kg^584.0μg/kg。结论本法具有简便、经济、灵敏、准确的特点,适宜在基层检测机构推广使用。Objective To establish a method for determination of zearalenone in the beer by high - performance liquid chromatography （HPLC） with UV detection. Methods Zearalenone in beer was extracted with ethyl acetate,and the extractant was dried - up under nitrogen flow at 50-C waterbath,and the residue was dissolved in acetonitrile. After filtration,the solution was used for analysis. Zorbax SB -18 was used as a separation column,and the mobile phase consisted of methanol - 0. 020 mol/L ammonium acetate （75 ＋ 25）. The detection wavelength was set at 236nm. Results Zearalenone showed a good linear relationship at the range of 0 - 10.0μg/ml with r = 0. 99997, and the detection limit was 5.0μg/kg. The relative standard deviations （RSDs） were 2.3% - 2.7%. The rates of recovery were 86.0% - 100% with the average recovery of 93.6%. Zearalenone contents detected in beer samples were 51.6μg/kg - 584μg/kg. Conclusion The method is simple,economical, sensitive and accurate, and it is suitable for determination of zearalenone in beer.

关 键 词：玉米赤霉烯酮 高效液相色谱法 啤酒 

分 类 号：R115[医药卫生—公共卫生与预防医学]