牡荆叶挥发油对烟草甲的杀虫活性  

Chemical composition and insecticidal activities of Vitex negundo L. var. cannabifolia(Sieb. et Zucc.)Hand.-Mazz. essential oil against the cigarette beetle

作　　者：吴彦[1] 尤春雪[2] 田兆福[1] 郭姗姗[2] 刘绍华[1] 黄东业[1] 邓宾玲[1] 李志华[1] 白家峰[1] 杜树山[2] 

机构地区：[1]广西中烟工业有限责任公司技术中心,南宁530001 [2]北京师范大学中药资源保护与利用北京市重点实验室,北京100875

出　　处：《植物保护》2016年第5期97-102,109,共7页Plant Protection

基　　金：广西科学研究与技术开发计划课题(桂科攻15118004);北京市自然科学基金(7142093)

摘　　要：用水蒸气蒸馏法提取牡荆叶挥发油,并采用气相色谱-质谱联用法分析其化学成分,基于柱色谱和核磁共振方法分离并鉴定化合物。从牡荆叶挥发油中鉴定出47种化合物,主要成分为β-石竹烯(25.8%)、桉油精(9.5%)、石竹烯氧化物(7.3%)和乙酸松油酯(6.1%)。并对牡荆叶挥发油及其分离得到的β-石竹烯和桉油精进行了杀虫活性测定。结果表明牡荆叶挥发油对烟草甲(Lasioderma serricorne)成虫具有触杀毒性(LD50为25.30μg/头)和熏蒸毒性(LC50为13.12mg/L)。挥发油中的主要化合物β-石竹烯和桉油精对烟草甲均有一定的触杀毒性(LD50分别为41.75μg/头和15.58μg/头)。桉油精对烟草甲还显示出较强的熏蒸活性(LC50为5.18mg/L)。结果提示牡荆叶挥发油及其活性化合物可用于烟草甲的综合防治。In order to find and develop new botanical pesticides against tobacco storage pests,bioactivity screening was performed.The essential oil obtained from the leaves of Vitex negundo L.var.cannabifolia（Sieb.et Zucc.）Hand.-Mazz.was investigated by GC-MS.A total of 47 components of the oil were identified and the main components in the oil were found to beβ-caryophyllene（25.8%）and eucalyptol（9.5%）,followed by caryophyllene oxide（7.3%）and terpinyl acetate（6.1%）.After screening,the essential oil exhibited potential insecticidal activity.In the process of assay,the essential oil was found to possess strong contact toxicity against Lasioderma serricorne adults with a LD50 value of 25.30μg/adult and fumigant toxicity with a LC50 value of 13.12 mg/L air.β-caryophyllene and eucalyptol also possessed strong contact toxicity against L.serricorne with a LD50 value of 41.75 and 15.58μg/adult,respectively.Moreover,eucalyptol exhibited strong fumigant toxicity against the cigarette beetle with a LC50 value of 5.18 mg/L air.The results indicate that the essential oil of Vitex negundo L.and the two isolated compounds has potential to be developed into a natural insecticide/fumigant for control of the cigarette beetle.

关 键 词：牡荆叶 烟草甲 触杀活性 熏蒸活性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]