Genotoxicity of ferric oxide nanoparticles in Raphanus sativus：Deciphering the role of signaling factors,oxidative stress and cell death  

作　　者：Quaiser Saquib Mohammad Faisal Abdulrahman A.Alatar Abdulaziz A.Al-Khedhairy Mukhtar Ahmed Sabiha M.Ansari Hend A.Alwathnani Mohammad K.Okla Sourabh Dwivedi Javed Musarrat Shelly Praveen Shams T.Khan Rizwan Wahab Maqsood A.Siddiqui Javed Ahmad 

机构地区：[1]Zoology Department, College of Sciences, King Saud University, P.O. Box 245S, Riyadh 114S1, Saucli Arabia [2]A.R. Al-Jeraisy Chair for DNA Research, Zoology Department, College of Sciences, King Saud University, P.O. Box 2455, Riyadh 11451,Saudi Arabia [3]Department of Botany & Microbiology, College of Sciences, King Saucl University, P.O. Box 2455, Riyaclh 11451, Saudi Arabia [4]Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, AIigarh 202002, India [5]Baba Ohulam Shah Badshah Uniuersity, Rajouri 185131,Jammu and Kashmir, India [6]Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012, India

出　　处：《Journal of Environmental Sciences》2016年第9期49-62,共14页环境科学学报（英文版）

基　　金：funded by the National Plan for Science,Technology and Innovation(MAARIFAH);King Abdul Aziz City for Science and Technology,Kingdom of Saudi Arabia,award number 12-BIO2919-02

摘　　要：We have studied the genotoxic and apoptotic potential of ferric oxide nanoparticles（Fe_2O_3-NPs） in Raphanus sativus（radish）.Fe_2O_3-NPs retarded the root length and seed germination in radish.Ultrathin sections of treated roots showed subcellular localization of Fe_2O_3-NPs,along with the appearance of damaged mitochondria and excessive vacuolization.Flow cytometric analysis of Fe_2O_3-NPs（1.0 mg/m L） treated groups exhibited 219.5%,161%,120.4% and 161.4% increase in intracellular reactive oxygen species（ROS）,mitochondrial membrane potential（ΔΨm）,nitric oxide（NO） and Ca2＋influx in radish protoplasts.A concentration dependent increase in the antioxidative enzymes glutathione（GSH）,catalase（CAT）,superoxide dismutase（SOD） and lipid peroxidation（LPO） has been recorded.Comet assay showed a concentration dependent increase in deoxyribonucleic acid（DNA） strand breaks in Fe_2O_3-NPs treated groups.Cell cycle analysis revealed 88.4% of cells in sub-G1 apoptotic phase,suggesting cell death in Fe_2O_3-NPs（2.0 mg/m L） treated group.Taking together,the genotoxicity induced by Fe_2O_3-NPs highlights the importance of environmental risk associated with improper disposal of nanoparticles（NPs） and radish can serve as a good indicator for measuring the phytotoxicity of NPs grown in NP-polluted environment.We have studied the genotoxic and apoptotic potential of ferric oxide nanoparticles（Fe_2O_3-NPs） in Raphanus sativus（radish）.Fe_2O_3-NPs retarded the root length and seed germination in radish.Ultrathin sections of treated roots showed subcellular localization of Fe_2O_3-NPs,along with the appearance of damaged mitochondria and excessive vacuolization.Flow cytometric analysis of Fe_2O_3-NPs（1.0 mg/m L） treated groups exhibited 219.5%,161%,120.4% and 161.4% increase in intracellular reactive oxygen species（ROS）,mitochondrial membrane potential（ΔΨm）,nitric oxide（NO） and Ca2＋influx in radish protoplasts.A concentration dependent increase in the antioxidative enzymes glutathione（GSH）,catalase（CAT）,superoxide dismutase（SOD） and lipid peroxidation（LPO） has been recorded.Comet assay showed a concentration dependent increase in deoxyribonucleic acid（DNA） strand breaks in Fe_2O_3-NPs treated groups.Cell cycle analysis revealed 88.4% of cells in sub-G1 apoptotic phase,suggesting cell death in Fe_2O_3-NPs（2.0 mg/m L） treated group.Taking together,the genotoxicity induced by Fe_2O_3-NPs highlights the importance of environmental risk associated with improper disposal of nanoparticles（NPs） and radish can serve as a good indicator for measuring the phytotoxicity of NPs grown in NP-polluted environment.

关 键 词：Ferric oxide nanoparticles Nanotoxicity DNA damage Apoptosis Oxidative stress Phytotoxicity 

分 类 号：X171.5[环境科学与工程—环境科学]