Effects of ZnO nanoparticles on perfluorooctane sulfonate induced thyroid-disrupting on zebrafish larvae  

作　　者：Jia Du Shutao Wang Hong You Zhongqiang Liu 

机构地区：[1]School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China [2]School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China [3]School of public health, Jia Mu Si Uniuersity, Jiamusi 154007, China [4]The Second Middle School of Funing at Qinhuangdao, Qinhuangdao 066300, China

出　　处：《Journal of Environmental Sciences》2016年第9期153-164,共12页环境科学学报（英文版）

基　　金：supported by the State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology)(No.2013DX09)

摘　　要：Perfluorooctane sulfonate（PFOS） and ZnO nanoparticles（nano-ZnO） are widely distributed in the environment.However,the potential toxicity of co-exposure to PFOS and nano-ZnO remains to be fully elucidated.The test investigated the effects of co-exposure to PFOS and nano-ZnO on the hypothalamic–pituitary–thyroid（HPT） axis in zebrafish.Zebrafish embryos were exposed to a combination of PFOS（0.2,0.4,0.8 mg/L） and nano-ZnO（50 mg/L）from their early stages of life（0–14 days）.The whole-body content of TH and the expression of genes and proteins related to the HPT axis were analyzed.The co-exposure decreased the body length and increased the malformation rates compared with exposure to PFOS alone.Co-exposure also increased the triiodothyronine（T3） levels,whereas the thyroxine（T4）content remained unchanged.Compared with the exposure to PFOS alone,exposure to both PFOS（0.8 mg/L） and nano-ZnO（50 mg/L） significantly up-regulated the expression of corticotropin-releasing factor,sodium/iodidesymporter,iodothyronine deiodinases and thyroid receptors and significantly down-regulated the expression of thyroid-stimulating hormone,thyroglobulin（TG）,transthyretin（TTR） and thyroid receptors.The protein expression levels of TG and TTR were also significantly down-regulated in the co-exposure groups.In addition,the expression of the thyroid peroxidase gene was unchanged in all groups.The results demonstrated that PFOS and nano-ZnO co-exposure could cause more serious thyroid-disrupting effects in zebrafish than exposure to PFOS alone.Our results also provide insight into the mechanism of disruption of the thyroid status by PFOS and nano-ZnO.Perfluorooctane sulfonate（PFOS） and ZnO nanoparticles（nano-ZnO） are widely distributed in the environment.However,the potential toxicity of co-exposure to PFOS and nano-ZnO remains to be fully elucidated.The test investigated the effects of co-exposure to PFOS and nano-ZnO on the hypothalamic–pituitary–thyroid（HPT） axis in zebrafish.Zebrafish embryos were exposed to a combination of PFOS（0.2,0.4,0.8 mg/L） and nano-ZnO（50 mg/L）from their early stages of life（0–14 days）.The whole-body content of TH and the expression of genes and proteins related to the HPT axis were analyzed.The co-exposure decreased the body length and increased the malformation rates compared with exposure to PFOS alone.Co-exposure also increased the triiodothyronine（T3） levels,whereas the thyroxine（T4）content remained unchanged.Compared with the exposure to PFOS alone,exposure to both PFOS（0.8 mg/L） and nano-ZnO（50 mg/L） significantly up-regulated the expression of corticotropin-releasing factor,sodium/iodidesymporter,iodothyronine deiodinases and thyroid receptors and significantly down-regulated the expression of thyroid-stimulating hormone,thyroglobulin（TG）,transthyretin（TTR） and thyroid receptors.The protein expression levels of TG and TTR were also significantly down-regulated in the co-exposure groups.In addition,the expression of the thyroid peroxidase gene was unchanged in all groups.The results demonstrated that PFOS and nano-ZnO co-exposure could cause more serious thyroid-disrupting effects in zebrafish than exposure to PFOS alone.Our results also provide insight into the mechanism of disruption of the thyroid status by PFOS and nano-ZnO.

关 键 词：nano-ZnO PFOS zebrafish thyroid hormone HPT axis 

分 类 号：X171.5[环境科学与工程—环境科学]