AMD3100联合地塞米松对造血干细胞的动员作用  

Combination effect of AMD3100 and dexamethasone on the mobilization of hematopoietic stem cells

作　　者：燕备战[1] 马会敏[1] 孔存权[1] 梁玉[1] 朱伟彦[1] 蒋舒婷[1] 

机构地区：[1]河南省人民医院输血科,郑州大学人民医院输血科,河南省郑州市450003

出　　处：《中国组织工程研究》2016年第36期5351-5357,共7页Chinese Journal of Tissue Engineering Research

基　　金：河南省科技攻关项目(142102310126)~~

摘　　要：背景:正常状态下外周血中的干细胞数量很低,将供者骨髓中的造血干细胞动员到外周血是造血干细胞移植的关键。目的:探索AMD3100和地塞米松联合用药对小鼠造血干细胞的动员效果,为临床应用奠定基础。方法:选用C57BL/6小鼠,单独或者联合注射AMD3100和地塞米松后采集外周血和骨髓造血干细胞,使用流式细胞仪鉴定外周血和骨髓中CD34^+细胞的含量,常规方法计数外周血白细胞含量,集落形成实验计数外周血和骨髓中造血干细胞粒-巨噬细胞混合集落形成单位。结果与结论:(1)AMD3100和地塞米松均可刺激外周血和骨髓中CD34^+细胞含量上升,分别在4 h和2 h时达到高峰,当联合使用AMD3100和地塞米松时,外周血和骨髓中CD34^+细胞含量上升幅度最大;(2)AMD3100或地塞米松均可刺激外周血中白细胞含量上升,AMD3100和地塞米松联合动员的外周血白细胞含量高于其他组;(3)AMD3100动员后外周血和骨髓中造血干细胞粒-巨噬细胞混合集落形成数量显著高于对照组,AMD3100和地塞米松联合动员的外周血和骨髓中造血干细胞粒-巨噬细胞混合集落形成数高于单用AMD3100或地塞米松动员组;(4)AMD3100或地塞米松能够动员造血干细胞从骨髓向外周血迁移,两者联合应用对小鼠造血干细胞动员效果优于单一药物。BACKGROUND： The number of hematopoietic stem cells in the peripheral blood is very low at normal physiological state. So it is critical to mobilize hematopoietic stem cells from donor＇s bone marrow into the peripheral blood. OBJECTIVE： To study the combination effect of AMD3100 and dexamethasone on the mobilization of hematopoietic stem cells in mice, thereby laying the foundation for clinical application. METHODS： C57BL/6 mice were injected with AMD3100 and dexamethasone alone or in combination. Then, hematopoietic stem cells in the peripheral blood and bone marrow were collected. CD34~＋ cell concentration in the peripheral blood and bone marrow was determined by flow cytometer and the content of leucocytes in the peripheral blood was counted by a normal method. The CFU-Mix colony formation ability of hematopoietic stem cells was identified by cell colony forming assay. RESULTS AND CONCLUSION： The concentration of CD34~＋ cells in the peripheral blood and bone marrow, the content of leukocytes in the peripheral blood and the CFU-Mix colony formation ability of hematopoietic stem cells were all improved by both AMD3100 and dexamethasone and especially their combined use. This indicates that both AMD3100 and dexamethasone could mobilize hematopoietic stem cells to migrate from the bone marrow to the peripheral blood, and when used in combination, the mobilization effect is better than that of single drug.

关 键 词：造血干细胞移植 地塞米松 造血干细胞动员 组织工程 干细胞 造血干细胞 AMD3100 动员 

分 类 号：R394.2[医药卫生—医学遗传学]