经肝动脉途径兔自体骨髓干细胞移植治疗肝硬化  

Autologous bone marrow stem cell transplantation for liver cirrhosis via the hepatic artery in rabbits

作　　者：王海舫[1] 刘洪霞[1] 邵菲[1] 贾蓓[1] 张岁[1] 周靖[1] 

机构地区：[1]河北医科大学第一医院肝病中心,河北省石家庄市050031

出　　处：《中国组织工程研究》2016年第36期5392-5397,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:如何使移植的骨髓干细胞更多的在肝内停留、分化是一个重要问题,也是经肝动脉途径移植骨髓干细胞治疗肝硬化的关键。目的:探讨经肝动脉途径移植自体骨髓干细胞对肝硬化的治疗效果。方法:将30只新西兰大白兔随机分为3组:正常对照组,干细胞移植组,模型对照组,每组10只。正常对照组不做任何处理,其余2组均建立肝硬化模型,干细胞移植组经肝动脉途径给予自体骨髓干细胞。在骨髓干细胞移植后的第1,2,4,8,10周分别检测肝功能指标,在第10周进行肝脏病理学检查。结果与结论:(1)骨髓干细胞移植第10周,干细胞移植组血清谷丙转氨酶、总胆红素、谷草转氨酶水平有所降低,活化部分凝血激酶时间明显减短,白蛋白水平升高,与模型对照组比较,差异有显著性意义(P<0.05);(2)干细胞移植组肝细胞形态完整,没有明显的水肿,假小叶结构仍然存在,与模型对照组比较,肝组织的纤维化程度明显减轻;(3)结果表明,经肝动脉途径移植自体骨髓干细胞可在短时间内提高体内白蛋白含量,改善机体肝功能,对肝硬化具有一定的治疗作用。BACKGROUND： How to make more transplanted bone marrow stem cells stay and differentiate in the liver is an important issue, which is also crucial for treatment of liver cirrhosis via the hepatic artery. OBJECTIVE： To investigate the therapeutic effect of autologous bone marrow stem cell transplantation via the hepatic artery on liver cirrhosis. METHODS： Thirty New Zealand white rabbits were equivalently randomized into normal control, stem cell transplantation and model groups. Animal models of liver cirrhosis were made in the latter two groups. Then, model rabbits in the stem cell transplantation group were subjected to autologous bone marrow stem cell transplantation via the hepatic artery. Liver function of rabbits was detected in 1, 2, 4, 8, 10 weeks after cell transplantation, and pathological detection of the liver was performed in the 10 th week. RESULTS AND CONCLUSION： At 10 weeks after cell transplantation, the liver function of the rabbits was improved significantly compared with the model group, including reduced activities of serum alanine aminotransferase, total bilirubin and aspartate aminotransferase, shortened activated partial thromboplastin time, and increased albumin level（P〈 0.05）. Pathological examination of the liver showed that the liver cells in the stem cell transplantation group were intact with no obvious edema and still had the structure of the pseudolobule, and compared with the model group, the degree of liver fibrosis was significantly reduced in the stem cell transplantation group. Our experimental results show that the transplantation of autologous bone marrow stem cells via the hepatic artery has a certain therapeutic effect on liver cirrhosis by increasing the body albumin content in a short time and improving the liver function.

关 键 词：肝硬化 骨髓祖代细胞 干细胞移植 肝动脉 组织工程 干细胞 移植 细胞移植 自体骨髓干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]