心脏干细胞对心肌细胞凋亡的保护机制  

Protective mechanism of cardiac stem cells against myocardial apoptosis

作　　者：高学东 胡科[2] 魏虎[3] 

机构地区：[1]南阳市第三人民医院心血管内科,河南省南阳市473000 [2]南阳市中心医院神经康复科,河南省南阳市473000 [3]南阳市第三人民医院老年病科,河南省南阳市473000

出　　处：《中国组织工程研究》2016年第36期5405-5411,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:心肌细胞凋亡会导致多种心脏疾病的出现,常规治疗大多无法获得理想的效果。心脏干细胞相关理论的出现,颠覆以往心肌细胞不可再生的观点,为各种心脏疾病的治疗提供了新思路。目的:探讨心脏干细胞在心肌细胞凋亡中的保护机制。方法:取5只新生大鼠进行心脏干细胞分离与培养,另取20只Wistar大鼠制备心肌梗死模型,然后随机分为2组,每组10只,分别设为观察组和对照组,建模后1 d,分别予以心脏干细胞与培养液注射。治疗后14 d,计算两组的心肌细胞凋亡指数,并检测两组心肌细胞凋亡相关蛋白表达情况。结果与结论:(1)细胞形态:连续培养3 d,组织块呈现出完全铺展生长状态,组织块中爬出少量的类纤维细,磁珠分离后进行传代培养5 d后,可观察到细胞重新聚集成团;(2)心肌细胞凋亡情况及相关蛋白表达:观察组的心肌细胞凋亡指数、Fas蛋白吸光度值和Fasl蛋白吸光度值均显著小于对照组(P<0.05),Bcl-2蛋白吸光度值显著大于对照组(P<0.05);(3)结果表明,通过对心肌梗死大鼠进行心脏干细胞移植治疗,可有效调节细胞凋亡相关蛋白的表达情况,抑制心肌细胞的凋亡。BACKGROUND： Myocardial apoptosis can lead to many kinds of heart diseases, but most of the conventional treatments cannot get desired effects. The emergence of cardiac stem cell related theory that subverts the previous view of myocardial cells provides a new idea for the treatment of a variety of heart diseases. OBJECTIVE： To investigate the protective mechanism of cardiac stem cells in myocardial apoptosis. METHODS： Cardiac stem cells were isolated from five neonatal rats. Another 20 Wistar rats were selected to make myocardial infarction models, and then model rats were equivalently randomized into observation and control groups. One day after modeling, rats in each group were given injection of cardiac stem cells or culture solution. Fourteen days after injection, myocardial apoptosis index was calculated and expression of apoptosis-related proteins was detected in both groups. RESULTS AND CONCLUSION： After continuous 3 days of culture, a completely spread growth in the cardiac tissues was visible, and a small amount of cells similar to fibrocytes climbed out from the cardiac tissue sample. These cells isolated using magnetic bead method were sub-cultured for 5 days, and found to be regrouped again. Compared with the control group, the apoptotic index, Fas and Fasl expression were significantly lower（P 〈0.05）, and Bcl-2 expression was significantly higher in the observation group（P 〈0.05）. All these findings show that cardiac stem cell transplantation can effectively regulate the expression of apoptosis-related proteins and inhibit the apoptosis of myocardial cells in rats with myocardial infarction.

关 键 词：干细胞 细胞凋亡 组织工程 移植 心脏干细胞 心肌梗死 心肌细胞 FASL蛋白 FAS蛋白 BCL-2蛋白 保护 大鼠 细胞分化 细胞移植 

分 类 号：R394.2[医药卫生—医学遗传学]