不同年龄脑出血大鼠海马齿状回神经干细胞增殖与分化差异的比较  

Comparative analysis of the proliferation and differentiation of neural stem cells in the hippocampal dentate gyrus of rats with different ages

作　　者：谢强[1] 王飞[1] 周国平[1] 张惠[2] 马进显[1] 

机构地区：[1]南阳市中心医院神经外科二病区,河南省南阳市473000 [2]南阳市中心医院耳鼻喉二病区,河南省南阳市473000

出　　处：《中国组织工程研究》2016年第36期5426-5431,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:脑出血后,海马齿状回的神经干细胞会被激活,导致其增殖分化能力的不断增强。通过内源性神经干细胞的不断分化和增殖,可以逐渐对老化、受损的神经元予以取代,进而发挥出保护脑结构等作用。目的:对比分析不同年龄脑出血大鼠的海马齿状回神经干细胞增殖分化差异情况。方法:成年和老年SD大鼠各96只,随机各自分为正常组(18只)、假手术组(18只)、脑出血模型组(60只)。其中,脑出血模型组制备脑出血模型,并进行BrdU标记,出血6 h、出血24 h、出血48 h、出血72 h、出血7 d,获得大鼠脑组织标本,计算脑含水量。术后3,7,14,21,28 d,进行BrdU/Neu N、Brd U/GFAP双标染色,计算双标阳性细胞数。结果与结论:(1)成年与老年大鼠脑出血组不同时间的脑含水量均显著高于正常组和假手术组(均P<0.05)。老年组正常组和假手术组的脑含水量均显著低于成年组(均P<0.05);(2)脑出血组不同时间的两侧BrdU阳性细胞数均显著高于正常组和假手术组(均P<0.05),且出血组不同时间的出血侧Brd U阳性细胞数均显著高于对侧(均P<0.05);(3)成年大鼠脑出血后海马齿状回神经干细胞免疫组织化学双标Brd U/Neu N和Brd U/GFAP结果显著高于正常组(均P<0.05);(4)成年大鼠3组脑出血后出血侧海马齿状回BrdU阳性细胞数均显著高于相应的老年组大鼠(均P<0.05);(5)结果表明,正常大鼠海马齿状回存在少量神经干细胞增殖现象,且老年大鼠的细胞增殖能力弱于成年大鼠。在发生脑出血后,大鼠的海马齿状回神经干细胞会出现增殖增强的情况,且老年大鼠的细胞增殖能力弱于成年大鼠。BACKGROUND： Cerebral hemorrhage can activate the proliferation and differentiation of neural stem cells in the dentate gyrus of the hippocampus. Through continuous differentiation and proliferation, endogenous neural stem cells can gradually replace aging and damaged neurons, thus protecting the brain structure.OBJECTIVE： To compare the difference of the proliferation and differentiation of neural stem cells in the dentate gyrus of the hippocampus of rats with different ages. METHODS： Ninety-six adult rats and 96 aged rats were randomly divided into normal group （n=18 per group）, sham operation group （n=12 per group） and cerebral hemorrhage group （model group, n=66 per group）, respectively. Cerebral hemorrhage models were made in the two model groups in which, the rats were subjected to cerebral hemorrhage for 6, 24, 48, 72 hours and 7 days, respectively. Then, brain tissues were collected to measure brain water content. BrdU/NeuN and BrdU/GFAP double staining were performed at 3, 7, 14, 21, 28 days after surgery to calculate the number of positive cells.RESULTS AND CONCLUSION： For both adult and aged rats, the brain water content was significantly higher than that in the normal group and sham operation group （P 〈 0.05）, while in the normal and sham operation groups, the brain water content was significantly lower in the aged rats than the adult rats （P 〈 0.05）. The number of bilateral BrdU-positive cells in the adult and aged model groups was significantly higher than that in the corresponding normal and sham operation groups （P 〈 0.05）, and moreover, the positive cell number at the hemorrhage side was significantly higher than that at the opposite side （P 〈 0.05）. In addition, the number of BrdU-positive cells at the hemorrhage side in the adult rats was significantly higher than that in the aged rats at different time after cerebral hemorrhage （P 〈 0.05）. Results from immunohistochemical double staining showed that the BrdU/NeuN and BrdU/GFAP expression in the

关 键 词：脑出血 干细胞 神经干细胞 海马 组织工程 海马齿状回 年龄 细胞增殖 细胞分化 

分 类 号：R394.2[医药卫生—医学遗传学]