上皮性卵巢癌中ALDH1和ABCG2的表达及其与微血管形成的关系  

Correlation between expression of ALDH1/ABCG2 and microvessel formation in epithelial ovarian cancer

作　　者：俞岚[1] 宋文庆[1] 周蕾[1] 武世伍[1] 王丹娜[1] 

机构地区：[1]蚌埠医学院第一附属医院病理科,蚌埠医学院病理学教研室,安徽省感染与免疫重点实验室,安徽蚌埠233000

出　　处：《中国病理生理杂志》2016年第10期1807-1814,共8页Chinese Journal of Pathophysiology

基　　金：高校省级优秀青年人才基金资助项目(No.2012SQRL094);蚌埠医学院自然科学类重点项目(No.BYKY1416ZD)

摘　　要：目的:本文旨在探讨上皮性卵巢癌(epithelial ovarian cancer,EOC)中乙醛脱氢酶1(ALDH1)、腺苷三磷酸结合盒转运体G2(ABCG2)蛋白表达及微血管密度(microvessel density,MVD)之间的关系。方法:收集198例EOC术后标本和60例卵巢良性上皮性肿瘤标本,应用免疫组织化学法检测EOC和卵巢良性上皮性肿瘤组织中ALDH1、ABCG2及CD105蛋白(微血管标志物)的表达情况。结果:在EOC和卵巢良性上皮性肿瘤组织中,ALDH1和ABCG2的阳性表达率分别为64.1%、61.6%和8.3%、6.7%;MVD分别为22.6±9.7和5.03±3.35,差异均有统计学显著性(P<0.05);ALDH1和ABCG2的表达与EOC的组织学分化、FIGO分期、腹腔器官及淋巴结转移有关(P<0.05),MVD与EOC的组织学分化、FIGO分期、腹腔器官及淋巴结转移和腹水形成有关(P<0.05);MVD分别与ALDH1和ABCG2的表达呈正相关(P<0.01),同时,ALDH1和ABCG2的表达亦呈正相关(P<0.01)。Kaplan-Meier生存分析表明,ALDH1和ABCG2的过表达以及MVD的增加均与患者的生存率有关,ALDH1和ABCG2表达阳性及MVD≥23的患者生存率明显低于ALDH1和ABCG2表达阴性及MVD<23的患者(P<0.05)。多因素分析显示FIGO分期、ALDH1表达、ABCG2表达和MVD是影响EOC根治术后患者预后的独立因素(P<0.05)。结论:EOC组织中ALDH1和ABCG2过表达以及MVD与肿瘤的分化程度、转移和预后等因素相关,这些指标的联合检测可能作为对EOC的进展及患者预后判断的重要指标。AIM：To elucidate the correlation between the expression of aldehyde dehydrogenase 1 （ALDH1）/ATP-binding cassette subfaminly G member 2 （ ABCG2 ） and microvessel density （ MVD ） in epithelial ovarian cancer （ EOC） .METHODS：In 198 specimens of EOC and 60 specimens of ovarian benign epithelial tumor tissues , the protein expression of ALDH1/ABCG2 and CD105 （ microvessel marker ） was detected by immunohistochemical staining .RESULTS：The positive rates of ALDH1 and ABCG2 in the EOC were 64.1%and 61.6%, respectively , while the positive rates in benign epithelial tumor tissues were 8.3%and 6.7%, respectively , and there were significant differences between them （P〈0.05）.In EOC and benign epithelial tumor tissues , the MVD were 22.6±9.7 and 5.03 ±.35, respectively, and the difference was also significant （P〈0.05）.The expression of ALDH1 and ABCG2 in EOC was significantly related to differentiation, FIGO stage,and abdominal organ and lymph node metastasis （P〈0.05）.MVD had correlation with differentiation, FIGO stage, ascite, and abdominal organ and lymph node metastasis （P〈0.05）.MVD had positive correlation with the expression of ALDH1 and ABCG2 （P〈0.01）.There was also a positive correlation between the expression of ALDH1 and ABCG2 （ P〈0.01） .Overexpression of ALDH1/ABCG2 and MVD≥23 were related to the poor prognosis .＆amp;nbsp;The survival rates in ALDH1/ABCG2 positive and MVD≥23 groups were significantly lower than those in ALDH 1/ABCG2 negative and MVD〈23 groups （P〈0.05）.The FIGO stage, the expression of ALDH1/ABCG2 and MVD were independent prognosis factors of EOC （ P〈0.05 ） .CONCLUSION： The results suggest that the expression of ALDH 1/ABCG2 and MVD in EOC are related to differentiation , lymph node metastasis , clinical stage and prognosis .Combined detection of these indexes may play an important role in predicting the progression and prognosis of EOC .

关 键 词：上皮性卵巢癌 乙醛脱氢酶1 腺苷三磷酸结合盒转运体G2 微血管密度 预后 

分 类 号：R737.31[医药卫生—肿瘤] R363[医药卫生—临床医学]