依维莫司对大鼠实验性IgA肾病的作用  

Everolimus prevents experimental IgA nephropathy in rats

作　　者：周敏[1] 唐惠玲[1] 

机构地区：[1]江苏食品药品职业技术学院药学院,江苏淮安223003

出　　处：《中国病理生理杂志》2016年第10期1887-1891,共5页Chinese Journal of Pathophysiology

摘　　要：目的:观察依维莫司对大鼠实验性Ig A肾病的作用并初步探讨其机制。方法:建立实验性Ig A肾病大鼠模型,实验共分为正常对照(control)组、模型组(Ig A组)和依维莫司给药组;测定给药后各组大鼠尿红细胞、尿蛋白和尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)的含量;免疫荧光染色检测肾组织中Ig A沉淀情况;Western blot法检测髓样分化因子88(My D88)、TLR4、NF-κB、IL-4和IL-13的蛋白表达水平;实时荧光定量PCR检测IL-4与IL-13的mRNA表达水平。结果:依维莫司能够抑制实验性Ig A肾病大鼠尿红细胞、尿蛋白和尿NAG含量的升高,抑制My D88、TLR4、NF-κB和IL-4和IL-13蛋白表达水平的上调,以及抑制IL-4和IL-13 mRNA表达水平的上调。结论:依维莫司能降低Ig A肾病大鼠尿红细胞、尿蛋白和尿NAG含量,其作用可能与其调节My D88、TLR4、NF-κB、IL-4与IL-13的表达有关。AIM：To investigate the effects of everolimus on the experimental IgA nephropathy in rats and its possible mechanisms .METHODS：The rat model of experimental IgA nephropathy was established .The rats were randomly divided into control group , IgA group and everolimus treatment group .After the corresponding treatments were given, urinary red blood cells , protein and N-acetyl-β-D-glucosaminidase （ NAG） were examined .Immunofluorescence staining was used to analyze the level of IgA precipitation in the renal tissues .Additionally, the protein expression of myeloid differentiation factor 88 （MyD88）, TLR4, NF-κB, IL-4 and IL-13 was determined by Western blot.The mRNA levels of IL-4 and IL-13 were detected by qPCR .RESULTS：Everolimus significantly inhibited the increases in the urinary levels of red blood cells, protein and NAG in experimental IgA nephropathy rats .Furthermore, IgA nephropathy-induced increases in the protein expression of MyD88, TLR4, NF-κB, IL-4 and IL-13 were attenuated after everolimus treatment .Similar results were obtained in the mRNA levels of IL-4 and IL-13 by qPCR detection .CONCLUSION： Everolimus improves the impairments of renal function in experimental IgA nephropathy rats as evidenced by decreasing urinary red blood cells , protein and NAG, which may be related to the inhibition of MyD 88, TLR4, NF-κB, IL-4 and IL-13 expression.

关 键 词：依维莫司 IGA肾病 髓样分化因子88 NF-κB TLR4 IL-4 IL-13 

分 类 号：R363[医药卫生—病理学]