FRAS1蛋白与非小细胞肺癌脑转移的关系  

Relationship between FRAS1 protein and brain metastases of NSCLC

作　　者：秦岭[1,2] 葛蒙晰[1] 周鑫莉[1] 黄若凡[1] 詹琼[1] 季笑宇 赵月华[1,3] 梁晓华[1] 

机构地区：[1]复旦大学附属华山医院肿瘤科,上海200040 [2]芜湖市第二人民医院,安徽芜湖241000 [3]复旦大学上海医学院肿瘤学系,上海200032

出　　处：《中国病理生理杂志》2016年第10期1892-1895,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81302010);上海市科委自然科学基金资助项目(No.13ZR1405000)

摘　　要：目的:探讨FRAS1蛋白与非小细胞肺癌(NSCLC)脑转移的关系。方法:采用q PCR检测FRAS1的mRNA在NSCLC脑转移组织和同期原发灶组织中的表达水平,采用SP免疫组化法检测FRAS1蛋白在肺癌组织和癌旁非肿瘤组织的表达水平,以及有脑转移和无脑转移NSCLC原发灶组织中FRAS1蛋白的表达水平。结果:FRAS1 mRNA在肺癌脑转移灶中的表达量是肺癌原发灶中的近10倍,差异具有统计学显著性(P<0.05);FRAS1蛋白在肺癌组织内表达,但在癌旁非肿瘤组织内未见表达;FRAS1蛋白在有脑转移NSCLC患者的肺癌组织中表达明显高于无脑转移NSCLC患者的肺癌组织,差异具有统计学显著性(P<0.01)。结论:NSCLC组织中FRAS1蛋白表达可能与肺癌发生有关,同时肺癌组织中FRAS1蛋白高表达可能与肺癌脑转移密切相关。AIM： To explore the relationship between FRAS 1 protein and brain metastases of non-small cell lung cancer （NSCLC）.METHODS：The mRNA expression of FRAS1 in the brain metastatic tumor tissues and primary tumor tissues of NSCLC was detected by qPCR .The protein expression of FRAS 1 in the tumor tissues and normal tissues adjacent to tumor tissues of NSCLC was measured by SP method of immunohistochemistry .The protein expression of FRAS 1 in NSCLC primary tumor tissues with or without brain metastases was also determined .RESULTS：The mRNA expression of FRAS1 in the brain metastatic zone was nearly 10 times higher than that in the primary tumor tissues , and there was significant difference between the 2 groups （P〈0.05）.FRAS1 protein was expressed in the NSCLC primary tumor tissues , but was not found in the normal tissues adjacent to primary tumor tissues .The protein expression of FRAS 1 in the NSCLC with brain metastases was significantly higher than that without brain metastases （ P〈0.01 ） .CONCLUSION： FRAS1 protein may be associated with the occurrence of NSCLC .The overexpression of FRAS1 protein may be related to brain metastases with NSCLC .

关 键 词：非小细胞肺癌 脑转移 细胞外基质 FRAS1 

分 类 号：R734.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]