miR-203在舌癌组织中的表达及其对Tca8113细胞活力和侵袭的影响  

Expression of miR-203 in human tongue carcinoma tissues and its influence on viability and invasion ability of Tca8113 cells

作　　者：郑红[1] 张文玲[2] 林波 张赛男[3] 刘瑞敏[3] 薛鹏[3] 

机构地区：[1]河南大学医学院病理生理学教研室,河南开封475004 [2]河南大学淮河临床学院口腔科,河南开封475004 [3]河南大学医学院免疫学教研室,河南开封475004

出　　处：《中国病理生理杂志》2016年第10期1896-1899,1904,共5页Chinese Journal of Pathophysiology

基　　金：开封市科技发展计划项目(No.1503112);河南大学科研基金重点项目(No.2010ZRZD02)

摘　　要：目的:研究舌癌组织中miR-203的表达,分析它与临床病理学参数之间的相关性,并探讨其与人舌癌Tca8113细胞活力及侵袭能力的关系。方法:收集28例舌癌手术标本,每例标本分别取癌灶及远端5 cm处正常黏膜组织(即远癌正常组织)2个部分。实时定量PCR检测舌癌患者肿瘤组织中miR-203的相对表达水平,分析其与临床病理特征之间的关系;脂质体2000介导miR-203 mimic和无关序列对照转染Tca8113细胞株,并通过CCK-8实验和Transwell小室侵袭实验检测上调miR-203对Tca8113细胞活力及侵袭能力的影响。结果:28例舌癌组织中miR-203表达水平与癌旁正常组织相比显著降低(P<0.05),miR-203的表达与肿瘤TNM分期及淋巴结转移存在相关性(P<0.05),而与患者的年龄和性别无关。Tca8113细胞转染miR-203 mimics上调miR-203表达可显著抑制Tca8113细胞株的活力及侵袭能力,与对照组比较差异有统计学显著性(P<0.05)。结论:上调miR-203表达能有效抑制舌癌细胞的生长和侵袭,miR-203可能成为舌癌基因治疗的靶点。AIM：To study the expression of miR-203 in tongue carcinoma tissues and the effect of miR-203 over-expression on the viability and invasion ability of Tca 8113 cells.METHODS：Twenty-eight pairs of tongue carcinoma tissues and adjacent nontumor tissues were collected , and the clinicopathological characters were analyzed .miR-203 was detected in the tongue tissues of 28 patients with tongue carcinoma by real-time PCR.miR-203 mimics and scramble were transfected into Tca8113 cells by Lipofectamine 2000.The expression of miR-203 was detected in Tca8113, Tca8113-miR-203 mimics and Tca8113-scramble cells by RT-qPCR.The cell viability was measured by CCK-8 assay.The cell invasion ability was determined by Transwell chamber invasion experiment .RESULTS：miR-203 expression was significantly down-regulated in the tongue carcinoma tissues compared with those in the adjacent nontumor tissues .The expression of miR-203 was associated with TNM stage and lymph node metastasis .Up-regulation of miR-203 inhibited the viability and invasion ability of Tca8113 cells.CONCLUSION：miR-203 suppresses the growth and invasion of tongue carcinoma cells .miR-203 may be a potential therapeutic target for treating human tongue cancer .

关 键 词：miR-203 舌癌 细胞活力 细胞侵袭 

分 类 号：R739.86[医药卫生—肿瘤] R730.23[医药卫生—临床医学]