重庆烟区青枯雷尔氏菌生化变种及序列变种的特性研究  

Biovar and sequevar of Ralstonia solanacearum in tobacco growing areas in Chongqing

作　　者：刘颖[1] 唐元满 张淑婷[1] 郭兵[1] 谷纪涛 丁伟[1,2] 

机构地区：[1]西南大学植物保护学院,重庆市北碚区天生路2号400715 [2]重庆烟草科学研究所,重庆市北碚区天生路2号400715

出　　处：《中国烟草学报》2016年第4期75-82,共8页Acta Tabacaria Sinica

基　　金：中国烟草总公司重点项目(110201202002);中国烟草总公司重庆市公司重点项目(NY20130501070005)

摘　　要：为进一步明确重庆烟区烟草青枯菌的遗传及生化特性,采用生化变种分类标准及演化型复合PCR、系统发育进化分析对重庆10个烟草青枯病发生区县青枯雷尔氏菌的种下分化情况进行了研究。结果表明:46株烟草青枯雷尔氏菌均属于生化变种3和亚洲分支菌株(演化型Ⅰ)。内切葡聚糖酶基因(egl)和DNA修复蛋白基因(mutS)部分序列的系统发育学分析表明:46株供试菌株可聚类到青枯雷尔氏菌演化型Ⅰ的3个序列变种,分别为序列变种15、17和44,其中序列变种17和44为优势菌株。表明重庆烟草青枯雷尔氏菌具有一定的遗传分化和地理区域特性。46 isolates were assessed to explore the diversity of tobacco Ralstonia solanacearum in Chongqing by using biovar, phylotype specific multiplex PCR, and phylogenetic relationships（egl and mut S）. Results showed that all tested isolates belonged to biovar 3 and phylotype I. The phylogenetic neighbor-joining tree based on partial sequences of egl and mut S genes indicated that the tested isolates were clustered into phylotype I, and were further divided into 3 sequevars, namely sequevars 15, 17, and 44. Sequevar 17 and 44 were predominant. R. solanacearum strains on tobacco showed genetic differentiation to some extent, which provided basis for studying relationship between classification and geographic areas.

关 键 词：烟草青枯菌 生化变种 演化型 系统进化分析 内切葡聚糖酶基因 DNA修复蛋白基因 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]