荧光原位杂交(FISH)检测乳腺癌中HER-2基因状态与p53、Ki-67、TOPOⅡ表达的相关性  

HER-2 gene status and its correlation with expression of p53,Ki-67,TOPOⅡ in breast cancer

作　　者：巩雷[1] 陈萍[1] 程薇[1] 刘宪军[1] 刘学鑫 

机构地区：[1]吉林市中心医院病理科,吉林吉林132011

出　　处：《现代肿瘤医学》2016年第21期3382-3386,共5页Journal of Modern Oncology

基　　金：吉林省科技发展计划项目(编号:20130102083JC)

摘　　要：目的:研究乳腺癌中HER-2基因状态和p53、Ki-67、TOPOⅡ蛋白表达的相互关系,以及HER-2基因扩增与HER-2蛋白表达的相关性。方法:运用FISH和IHC技术分别检测172例浸润性乳腺癌中HER-2基因状态及HER-2、p53、Ki-67、TOPOⅡ蛋白表达情况,分析HER-2基因状态与其相互的关系。结果:172例浸润性乳腺癌中HER-2基因扩增与p53、Ki-67、TOPOⅡ蛋白表达呈正相关(P<0.05),HER-2基因扩增阳性率为43.6%(75/172),HER-2基因扩增与HER-2蛋白表达具有相关性(P<0.05)。结论:联合检测HER-2基因状态和p53、Ki-67、TOPOⅡ蛋白表达可为浸润性乳腺癌的预后及分子靶向治疗提供依据。Objective To study HER -2 gene status and its relationship with the protien expression of p53,Ki - 67,TOP〇II in breast cancer, and the correlation between HER -2 gene amplification and its protein expression. Methods： Detected the HER -2 gene status and the protein expression of HER -2 gene,p53,Ki -67,and TOPO II in 172 cases of invasive breast cancer,by FISH and IHC technique,and analyze the relationship between state of HER -2 and protein expression. Results： In 172 cases of invasive breast cancer,the HER - 2 gene amplification was posi-tively correlated with p53 , Ki - 67 ,TOPO II protein expression（ P 〈 0. 05 ） . The positive rate of HER - 2 gene amplifi-cation was 43. 6% （ 75/172） . And HER - 2 protein expression was correlated with HER - 2 gene amplification（ P 〈0. 05） . Conclusion ： Joint detection of HER -2 gene state and protein expression of p53 ,Ki -67 ,TOPO II could pro-vide basis for prognosis and molecular targeted therapy for invasive breast cancer.

关 键 词：乳腺癌 HER-2 P53 KI-67 TOPOⅡ 荧光分子原位杂交 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]