发芽糙米微量元素锌的生物强化  

Biofortification of Germinated Brown Rices with Exogenous Zinc Element

作　　者：胡广林[1] 胡晓梦[1] 朱莉[1] 李远颂[2] 

机构地区：[1]热带生物资源教育部重点实验室,海南大学材料与化工学院,海口570228 [2]海南大学研究生处,海口570228

出　　处：《广东微量元素科学》2016年第10期11-17,共7页Trace Elements Science

基　　金：国家自然科学基金项目(31460390)

摘　　要：目的 发芽糙米的锌生物强化培育及对人体补锌的适用性探讨。方法 选取3个品种（吉梗81号、博优209号、黑糯米）的糙米为材料,用不同质量浓度锌培养液（0-250μg·m L^-1）浸种24 h（28℃）并发芽36 h（30℃）,在萌发过程中实行锌的生物强化,培育富锌发芽糙米。通过测定不同锌含量条件下发芽糙米的萌发参数（吸水率、发芽率）,以及锌、Vc和矿物质（Fe、Mn、Mg）含量,探明培养液锌的适宜含量条件和富锌发米糙米的补锌适用性。结果 对吉梗81号、博优209号、黑糯米等3个品种,富锌发芽糙米培育的锌培养液适宜质量浓度均为75-150μg·m L^-1,最佳质量浓度为100μg·m L^-1。3个品种发芽糙米对锌的富集能力由强到弱顺序为：吉梗81号,博优209号,黑糯米。与对照处理（CK）相比,富锌发芽糙米Vc、GABA含量较高,而矿质元素（Fe、Mn、Mg）渗漏损失略低。富锌发芽糙米每10 g（DW）的锌积累量,对吉梗81号、博优209号及黑糯米等3个品种可提供锌占中国成人RNI百分数分别是：53.8%（男性）和89.7%（女性）,45.6%（男性）和76.0%（女性）,以及41.1%（男性）和68.5%（女性）。结论 发芽糙米可能是进行锌生物强化的优良载体,预计富锌发芽糙米适用于人体补锌。Objective To explore the cultivating zinc - enriched germinated brown rices by biolortification and its applicability in zinc supplementation for human body. Methods Zinc - enriched germinated brown rices were cultivated from rice seeds （three varieties ： Jigeng No. 81, Boyou No. 209 and Black Glutinous Rice） by biofortification during germination with zinc cultures （0 -250 mg/L） at a 24 hour seed soaking time （28 ℃）and a 36 hour cultivation time. Appropriate concentration of zinc germinated brown rices were ascertained in terms of germination parameters （ percentage of water absorption , percentage of seed germination）, contents of zinc , Vc , GABA, and minerals （ Fe,Mn and Mg） in germinated brown rices. Results The appropriate zinc concentration in cultures was in the equal range of 75 ？150 mg/L for Jigeng No. 81,Boyou No. 209 and Black Glutinous Rice, with the optimum concentration at 100 mg/L. The zinc enrichment ability of germinated brown rices for the varieties is in the following order： Jigeng No. 81 〉 Boyou No. 209 〉 Black Glutinous Rice. Contents of Vc and GABA in zinc - enriched germinated brown rices were higher than those in the control （CK）,while leakage percentages of minerals （ Fe, Mn and Mg） in zinc - enriched germinated brown rices were slightly lower than those in CK. The percentages of zinc accumulation amounts in zinc - enriched germinated brown rices accounting for the recommended nutrient intake （ RNI） of zinc for Chinese adults were as follows： 53. 8% （male） and 89. 7% （female） for Jigeng No. 81, 45. 6% （male） and 76. 0% （female） for Boyou No. 209, as well as 41. 1% （male） and 68.5% （female） for Black Glutinous Rice. Conclusion Germinated brown rices may be a good vehicle for zinc biofortification. It is expected that zinc - enriched germinated brown rices will be utilized for the zinc supplementation for Zn - deficient populations.

关 键 词：发芽糙米 锌 生物强化 萌发 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程] Q945.34[轻工技术与工程—食品科学与工程]