上皮细胞粘附分子在肝动脉化疗栓塞治疗肝癌中的表达改变及其功能  

The influence and alteration of EpCAM expression in TACE treated hepatocellular carcinoma

作　　者：楼敏[1] 白文林[1] 常秀娟[1] 刘泽[1] 李因茵[1] 荣光华[1] 曾珍[1] 

机构地区：[1]解放军第三〇二医院肝脏肿瘤诊疗与研究中心,北京100039

出　　处：《传染病信息》2016年第4期209-212,221,共5页Infectious Disease Information

基　　金：军队"十二五"面上课题(CWS12J024);国家自然基金面上课题(81372248)

摘　　要：目的明确肝动脉化疗栓塞(transcatheter hepatic arterial chemoembolization,TACE)治疗对肝细胞癌(hepatocellular carcinoma,HCC)肿瘤组织中肿瘤干细胞指示分子——上皮细胞粘附分子(epithelial cell adhesion molecule,Ep CAM)表达及肝癌干细胞生物学行为的影响。方法用实时PCR分别检测30例经TACE治疗和30例未经TACE治疗的HCC患者肿瘤组织中Ep CAM的表达水平。在此基础上,用Ep CAM的si RNA载体,在HCC细胞系Hep G2和高侵袭性的MHCC-97H中检测Ep CAM对抗肿瘤药物作用的影响;在MHCC-97H细胞系中,检测降低Ep CAM表达对MHCC-97H侵袭作用的影响。结果接受TACE治疗的HCC患者肿瘤组织中的Ep CAM表达水平升高(P<0.05)。与对照组相比,在Hep G2细胞中转染Ep CAM的si RNA能够显著上调Hep G2细胞对分子靶向抗肿瘤药物索拉非尼以及细胞毒性化疗药物奥沙利铂和表柔比星的敏感性。在MHCC-97H中转染Ep CAM的si RNA能够显著抑制其侵袭作用。结论 TACE治疗可引起HCC组织中Ep CAM的表达水平明显升高;降低HCC细胞中Ep CAM表达水平,可导致HCC细胞的肿瘤干细胞特征如侵袭性显著下降,对抗肿瘤药物的敏感性升高。Objective To determine whether transcatheter arterial chemoembolization（TACE） treatment modulates（cancer stem cell, CSC） marker expression and the detailed function of Ep CAM in HCC cells via its si RNA. Methods The expression levels of Ep CAM were detected by q PCR in 30 HCC patients with TACE treatment and 30 HCC patients without TACE treatment, respectively. On this basis, we detected the effect to anti-tumor agents action of Ep CAM in HCC cell lines Hep G2 and high invasive MHCC-97 H by si RNA carrier of Ep CAM. Next, the si RNA of Ep CAM was transfected to determine whether Ep CAM modulates HCC cells＇ in vitro invasion and the sensitivity to anti-tumor agents. Results Ep CAM expression levels in tumor tissues of HCC patients treated with TACE were significantly higher（P〈0.05）.In contrast to the control group, Transfection of Ep CAM si RNA enhanced the sensitivity of Hep G2 to sorafenib, oxaliplatin and epirubicin. In addition, Ep CAM si RNA also disrupted the invasion of MHCC-97 H cells. Conclusions TACE can increase Ep CAM expression level in HCC significantly; Reduce of Ep CAM expression levels in HCC cells can lead to HCC cells of tumor stem cell characteristics such as invasion significantly decreased, and increase sensitivity to chemotherapeutic drugs.

关 键 词：肝细胞癌 EPCAM TACE治疗 肿瘤干细胞 

分 类 号：R735.7[医药卫生—肿瘤]