脂滴在小鼠酒精性肝损伤过程中的表达变化  

Expression changes of lipid droplets during alcoholinduced liver injury in mice

作　　者：卢华杰[1] 李三强[1] 张勇勇[1] 王善龙[1] 乔新杰[1] 霍旭磊 李小苹[1] 侯松林[1] 

机构地区：[1]河南科技大学医学院,肝损伤与修复分子医学重点实验室,河南省洛阳市471003

出　　处：《世界华人消化杂志》2016年第25期3683-3688,共6页World Chinese Journal of Digestology

基　　金：河南省高校科技创新人才支持计划资助项目,No.13HASTIT025;河南科技大学创新团队资助项目,No.2015XTD003~~

摘　　要：目的探究小鼠酒精性肝损伤中的病理表现.方法健康昆明小鼠♂30只,随机分为对照组(n=10),模型组(n=20),对照组于0 wk处死,取肝脏;模型组进行适应性喂养1 wk后,给予56度白酒灌胃(0.15 m L/20 g·d),连续8 w k,于4 wk和8 wk,颈椎脱臼处死小鼠,取其肝脏待用,制备石蜡切片和冰冻切片,采用HE染色和油红O染色,观察酒精性肝损伤中的脂滴表达变化,采用Image-Pro Plus6.0对病理切片样本进行累计光密度(integral optical density,IOD)定量分析.结果与对照组比较,第4周脂滴含量开始增加(20.29±7.07 vs 8.06±2.06,P<0.01),第8周末脂滴含量明显增多(34.88±15.33 vs 8.06±2.06,P<0.01),8 wk与4 wk相比,脂滴的表达量呈上升趋势(34.88±15.33 vs 20.29±7.07,P<0.05).结论 8 wk末成功建立了酒精性脂肪肝模型,可以用来评估酒精性脂肪肝治疗药物的预后作用;在小鼠酒精性肝损伤中,随着脂肪变性的发生,脂滴含量不断增加.AIM To investigate the pathological changes in the process of alcoholic liver injury.METHODS Thirty healthy male Kunming mice were randomly divided into a control group（n =10） and a model group（n = 20）.The control group was executed to take the liver tissue at 0 wk.The model group was treated with alcohol of 56 degrees（0.15 mL/20 g·d） for8 wk,and then executed to take the liver tissue at four and 8 wk.The expression changes of lipid droplets during alcoholic liver injury were assessed by hematoxylin and eosin staining and oil red O staining.The integral optical density of histology samples was analyzed with ImageProPlus6.0 software.RESULTS The content of lipid drops began to increase at 4 wk（20.29 ± 7.07 vs 8.06 ± 2.06,P 0.01）,and significantly increased at 8 wk（34.88 ±15.33 vs 8.06 ± 2.06,P 0.01）.Compared to that at 4 wk,the amount of expression of lipid droplets showed a rising trend at 8 wk（34.88± 15.33 vs 20.29 ± 7.07,P 0.05）.CONCLUSION The levels of lipid droplets show a growing trend along with the aggravation of hepatic steatosis during alcoholic liver injury in mice.

关 键 词：小鼠 酒精性肝损伤 油红染色 苏木素-伊红染色 

分 类 号：R575[医药卫生—消化系统]