小麦×玉米杂交双单倍体后代的HMW-GS组成和易位分析  

Analysis of HMW-GS and Translocation Line in Progenies of Doubled Haploid from Wheat × Maize Cross

作　　者：庞建周[1] 王雪征[1] 茜晓哲[1] 王晨阳[1] 陈淑萍[1] PANG Jianzhou WANG Xuezheng QIAN Xiaozhe WANG Chenyang CHEN Shuping(Dry Land Farming Institute, Hebei Academy of Agriculture and Foresty Sciences, Key Lab of Crop Drought Tolerance Research of Hebei Province,Hengshui 053000,Chin)

机构地区：[1]河北省农林科学院旱作农业研究所,河北省农作物抗旱研究重点实验室,河北衡水053000

出　　处：《华北农学报》2016年第4期126-129,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省农林科学院前期预备项目(A2015040301);河北省农林科学院旱作农业研究所青年基金项目

摘　　要：为了提高优质小麦的育种效率,在早代明确高分子量谷蛋白亚基和醇溶蛋白的组成,增加育种的针对性,以1个小麦杂交组合的296个DH株系群体为试材,采用半粒SDS-PAGE法检测麦谷蛋白,且用A-PAGE检测了醇溶蛋白。结果表明:母本衡09-6324的HMW-GS构成为null、7+9、2+12,是有Sec-1特征带的1BL/1RS易位系品种,父本师栾02-1亚基组成1、7+9、5+10,是非1BL/1RS易位系品种。296个DH株系共鉴定出6种不同的HMW-GS类型,均为父母本表达亚基类型的再分配,无变异类型。与面包加工品质呈正相关的5+10亚基出现频率为35.13%。DH株系材料中1BL/1RS易位系类型的占54.05%,1、5+10优质亚基同时出现,且没有Sec-1表达的占15.20%,这些株系可作为新的面包小麦种质资源使用。采用半粒SDS-PAGE和A-PAGE法对育种早代的优异单株进行有效鉴定和选择,可以提高优质小麦育种效率。To improve the efficiency of high quality wheat breeding program,clarify the composition of HMWGS and Gliadin in the early generation,296 lines from the wheat maize cross were analyzed useing SDS-PAGE method in this experiment,along with this Gliadin were analyzed useing A-PAGE method. A statistics showed that female parent Heng 09-6324 carried null,7 ＋ 9,2 ＋ 12 subunits,and were 1BL/1RS translocation lines with Sec-1 characteristic band. Male parent Shiluan 02-1 carried 1,7 ＋ 9,5 ＋ 10 subunits,and were non 1BL/1RS translocation. Six types of HMW-GS all originated from parents were identified from 296 DH lines. The frequency of subunits 5 ＋ 10 which positively related to bread baking quality was 35. 13%. A statistics showed that in the A-PAGE the type of translocation of the DH lines accounted for 54. 05%,and the percent of lines with subunits 1 and 5 ＋ 10,but non Sec-1 translocation were 15. 20%,these lines could be used as new germplasm for quality breeding. The paper indicated that that the method of half-seed SDS-PAGE and A-PAGE which could be used to identify and select excellent plants in early generation breeding effectively.

关 键 词：小麦 玉米 双单倍体 半粒电泳法 HMW-GS 黑麦碱 

分 类 号：S512.03[农业科学—作物学] S513.03