蓝刺头多糖体外抗氧化及抗菌活性的研究  

Study on Antioxidant and Antibacterial Activity in vitro of Echinops latifolius Tausch Polysaccharides

作　　者：杨斌[1] 萨茹丽[2] 张月梅[1] 达来宝力格[1] 宋越[1] 宋爱军[1] 陈伟[1] 卢岩[1] 刘敏[1] 杨英[3] 赵世华[1] YANG Bin SA Ruli ZHANG Yuemei DALAI Baolige SONG Yue SONG Aijun CHEN Wei LU Yan LIU Min YANG Ying ZHAO Shihua(Institute of Veterinary Medicine,Inner Mongolia Academy of Agricultural and Huhhot 010031 ,China College of Animal Science,Inner Mongolia Agricultural China College of Veterinary Medicine, Inner Mongolia Agricultural University Animal Husbandry Sciences, University, Huhhot 010018 , Huhhot 010018 ,China)

机构地区：[1]内蒙古农牧业科学院兽医研究所,内蒙古呼和浩特010031 [2]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018 [3]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《华北农学报》2016年第4期233-238,共6页Acta Agriculturae Boreali-Sinica

基　　金：内蒙古自治区自然科学基金项目(2016BS(LH)0301);内蒙古农牧业创新基金项目(2013CXJJM05)

摘　　要：通过研究蓝刺头粗多糖及其洗脱纯化组分蓝刺头多糖A、B、C的体外抗氧化和抗菌作用,初步确定蓝刺头多糖的生物活性。结果显示,添加浓度相同时,蓝刺头粗多糖的总还原能力以及清除DPPH有机自由基能力最强,显著高于蓝刺头多糖A,极显著高于蓝刺头多糖B、C。当添加浓度为0.1～0.3 mg/m L时,蓝刺头粗多糖清除羟基自由基的能力略高于蓝刺头多糖A,但差异不显著;当添加浓度为0.4 mg/m L时,蓝刺头粗多糖清除羟基自由基的能力略高于蓝刺头多糖A,蓝刺头多糖A略高于蓝刺头多糖B,三者差异不显著,但均极显著高于蓝刺头多糖C,当添加浓度为0.5 mg/m L时,蓝刺头粗多糖清除羟基自由基的能力显著高于蓝刺头多糖A、B,极显著高于蓝刺头多糖C。试验条件下,蓝刺头粗多糖和蓝刺头多糖A对大肠杆菌为低度敏感,最低抑菌浓度均为0.62 mg/m L,蓝刺头多糖B和C对大肠杆菌不敏感,最小抑菌浓度均为1.25 mg/m L;蓝刺头粗多糖、蓝刺头多糖A、B和C对金黄色葡萄球菌为不敏感,最低抑菌浓度分别为1.25,2.50,2.50,2.50 mg/m L;蓝刺头粗多糖、蓝刺头多糖B、C对铜绿假单胞菌为不敏感,最低抑菌浓度均为5.00 mg/m L,蓝刺头多糖A对铜绿假单胞菌无抑制作用;蓝刺头粗多糖和蓝刺头多糖A、B、C对沙门氏菌均为不敏感,最低抑菌浓度分别为2.50,2.50,1.25,5.00 mg/m L。说明蓝刺头粗多糖的抗氧化能力最强,其次为蓝刺头多糖A,而蓝刺头多糖B和C的抗氧化能力较弱。蓝刺头粗多糖和蓝刺头多糖A具有一定的抗大肠杆菌活性,蓝刺头粗多糖和蓝刺头多糖A、B、C均无抗金黄色葡萄球菌、铜绿假单胞菌以及沙门氏菌活性。In this study antioxidant activity and antibacterial activity of Echinops latifolius Tausch polysaccharides was researched. The result showed that when the initial concentrations were same,ETCP has a highest effect of total reducing capacity,clearance of DPPH free radical in vitro,higher than ETPA,significantly higher than ETPB and ETPC. When the added concentrations were 0. 1-0. 3 mg/m L,ETCP has a little higher scavenging effects on free radicals than that of ETPA,but no obvious difference; when the added concentrations were 0. 4 mg/m L,ETCP has a little higher scavenging effects on free radicals than that of ETPA,ETPA has a little higher scavenging effects than that of ETPB,and difference were not significant,they were significantly higher than that ETPC,when the added concentrations were 0. 5 mg/m L,ETCP has a higher scavenging effects on free radicals than that of ETPA,ETPB,significantly higher than ETPC. Experimental condition,ETCP and ETPA were less sensitive to E. coli,the mini-mal inhibitory concentration（ MIC） were 0. 62 mg/m L,ETPB and ETPC were insensitive the MIC were 1. 25 mg/m L;ETCP,ETPA,ETPB and ETPC were insensitive to Staphylococcus aureus,the MIC were 1. 25,2. 50,2. 50,2. 50 mg/m L respectively; ETCP,ETPB and ETPC were insensitive to Pseudomonas aeruginosa,the MIC were 5. 00 mg/m L,ETPA had no inhibition to Pseudomonas aeruginosa; ETCP,ETPA,ETPB and ETPC were insensitive to Salmonella,the MIC were 2. 50,2. 50,1. 25,5. 00 mg/m L respectively. It means ETCP has the strongest antioxidant effect and secondly for ETPA,ETPB and ETPC were weak. ETCP and ETPA has a certain resistance to activity of E. coli,and ETPA,ETPB and ETPC has no antibacterial action to Staphylococcus aureus,Pseudomonas aeruginosa and Salmonella.

关 键 词：蓝刺头 多糖 抗氧化活性 抗菌活性 

分 类 号：S853.74[农业科学—临床兽医学]