响应面试验优化高温高压制备玉米环(组氨酸-脯氨酸)二肽关键工艺  

Optimization of Key Processing Parameters for Preparation of Corn Cyclo(His-Pro) by High Temperature/High Pressure Method

作　　者：樊红秀[1] 刘婷婷[1] 刘鸿铖[1] 任华华[1] 张艳荣[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品科学》2016年第20期6-12,共7页Food Science

基　　金："十二五"国家科技支撑计划项目(2013BAD16B08);国家高技术研究发展计划(863计划)项目(2011AA100805)

摘　　要：将高温高压技术应用于玉米环(组氨酸-脯氨酸)二肽(cyclo(His-Pro),CHP)的制备,以玉米蛋白水解物为原料,在水相溶液中采用高温高压法对其进行环化,合成CHP。通过单因素试验考察反应温度、底物质量浓度、反应时间和KHCO_3浓度对CHP提取量的影响,应用响应面试验设计对高温高压环化反应条件进行优化。结果表明最佳反应条件为反应温度125.9℃、反应压力0.25 MPa、底物质量浓度20.5 mg/m L、KHCO_3浓度0.16 mol/L、反应时间5.3 h,此条件下CHP提取量可达6.58 mg/g。采用超高效液相色谱-串联质谱法对水解物中的CHP进行进一步鉴定,结果表明制备的玉米CHP与预期结构相符。In this study, we investigated the synthesis of corn cyclo （His-Pro） （CHP） in aqueous solution by a hightemperature/pressure method from corn protein hydrolysate prepared in our laboratory. First of all, the one-factor-at-a-timemethod was used to investigate the influences of reaction temperature, substrate concentration, KHCO3 concentration andreaction time on CHP yield. Subsequently, the reaction conditions were optimized using response surface methodology.Results revealed that the optimum reaction conditions were determined as follows： reaction temperature, 125.9 ℃;reaction pressure, 0.25 MPa; substrate concentration, 20.5 mg/mL; KHCO3 concentration, 0.16 mol/L; and reaction time,5.3 h, resulting in the maximum yield of CHP as high as 6.58 mg/g. The prepared CHP exhibited the expected structure asconfirmed by ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）.

关 键 词：玉米蛋白 环(组氨酸-脯氨酸)二肽 高温高压法 环化反应 

分 类 号：TS213.4[轻工技术与工程—粮食、油脂及植物蛋白工程]