复合PCR荧光检测方法检测转基因水稻品系  

Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events

作　　者：张明哲 张晓峰 徐莉莉 陈笑梅 周圆 尹文秀 陈吴健 李孝军 

机构地区：[1]浙江省检验检疫科学技术研究院,浙江杭州310016 [2]舟山出入境检验检疫局,浙江舟山316000

出　　处：《食品科学》2016年第20期187-190,共4页Food Science

基　　金：浙江省重大科技专项(2011C12023);国家质检总局科技计划项目(2011IK249);浙江省公益技术项目(2011C22065;2014C32004)

摘　　要：建立一种高通量的转基因复合聚合酶链式反应(polymerase chain reaction,PCR)荧光检测方法,该方法通过对转基因检测内源、外源及品系基因检测引物进行荧光标记,经复合PCR扩增和毛细管电泳荧光检测,实现特异性强、高通量检测。结果表明,复合PCR荧光检测方法检测4种转基因水稻品系特异性好,并且灵敏度达到0.1%,可以在进出口检验检疫、食品安全监测等领域进行应用。In this research, a high-throughput and specific method for the detection of genetically modified （GM） rice eventswas developed using fluorescence multiplex PCR and capillary electrophoresis based on fluorescence-labeled primers forendogenous, exogenous and event-specific genes. The fluorescence multiplex PCR method could specifically detect fourGM rice events with a sensitivity of 0.1%. This method has the potential to be applied in the inspection and quarantine ofimport and export genetically modified commodities and in food safety detection.

关 键 词：复合聚合酶链式反应 毛细管电泳 转基因水稻 品系鉴定 

分 类 号：Q789[生物学—分子生物学]