NF-κB介导的促凋亡蛋白Bak表达在溃疡性结肠炎发病中的作用研究  

Research of roles of NF-κB and promote apoptosis protein Bak in ucelrative colitis

作　　者：陈晓[1] 王启之[1] 郦忆文[1] 马文静[1] 于东红[1] 

机构地区：[1]蚌埠医学院第一附属医院,蚌埠233000

出　　处：《中国免疫学杂志》2016年第9期1286-1290,共5页Chinese Journal of Immunology

摘　　要：目的:探讨核因子-κB(Nuclear factor kappa B,NF-κB)介导的促凋亡蛋白Bak(Bcl-2 associated K protein gene,Bak)及TNF-α的表达在溃疡性结肠炎(Ulcerative colitis,UC)发病中的作用机制。方法:选购80只清洁级SD大鼠,分为模型组和对照组。UC大鼠模型制造采用"三硝基苯磺酸+乙醇"方法,模型制造成功后观察、评估结肠黏膜的大体形态及组织学变化。用免疫组织化学及RT-PCR法检测模型组与对照组中的NF-κB、Bak、TNF-α的表达水平,并分析相互之间关系。结果:UC大鼠模型制造成功率为97%。模型组固有层和黏膜层内炎性细胞浸润较对照组明显增多(P<0.01);NF-κB、TNF-α蛋白及mRNA表达水平模型组较对照组明显升高(P<0.01);Bak蛋白在模型组的炎细胞中表达明显低于对照组(P<0.01),而在结肠黏膜上皮细胞中的水平与对照组无明显差异(P>0.05)。NF-κB、TNF-α蛋白阳性细胞百分数及mRNA水平随模型组的组织学等级升高而增强,而Bak蛋白阳性细胞百分数是减弱的(P<0.05);且模型组中NF-κB与Bak蛋白的表达水平呈负相关(r=-0.793,P<0.01),NF-κB与TNF-α蛋白表达成正相关(r=0.892,P<0.01)。结论:NF-κB介导的促凋亡蛋白Bak的表达参与了UC的发生、发展。Objective：To study the expression levels of nuclear factor kappa B,Bcl-2 associated K and TNF-α proteins to discuss the effects of NF-κB and Bak proteins in the pathogenesis of UC.Methods：Eighty clean grade of adult Sprague-Dawley（SD） rats were used,male and female in half and then rando mly selected sixty as the model group,another twenty as the control group.SD rats model were manufactured by a compound method：Trinitrobenzene sulfonic acid（TNBS） ＋ ethanol.We observed and assessed colonic mucosa by the general morphology and histological changes.To applicated immunohistochemistry and RT-PCR methods to detected the protein and mRNA expression levels of NF-κB,Bak and TNF-α in the model groups and the control group and to analysed their relationships.Results：The successful rate of making model was 97%.The number of inflammatory cells in the model groups more than the control（P〈0.01）.Group immunohistochemical and RT-PCR,NF-κB and TNF-α proteins and mRNA in UC colon epithelium cells and inflammatory cells were higher than the control（P〈0.01）.Bak protein in inflammatory cells were lower than the control（P〈0.01）,but there was no statistical significance in epithelial cells（P〉0.05）.The expression levels of NF-κB,TNF-α increased as the histological grade increased（P〈0.05）,however,the expression level of Bak decreased（P〈0.05）.NF-κB in colonic mucosa of rats with UC had a significantly positive correlation with that of TNF-α（r = 0.892,P〈0.01）,and negatively correlated with that of Bak（r =-0.793,P〈0.01）.Conclusion：The levels of NF-κB and Bak may be related to the occurrence and development of UC.

关 键 词：核因子-ΚB BAK 溃疡性结肠炎 

分 类 号：R574.1[医药卫生—消化系统]