乙基亚硝基脲构建β-地贫小鼠模型的初步研究  

Ethylnitrosourea-induced mouse model of β-thalassemia: a preliminary study

作　　者：何飞[1,2] 苏华[1] 韦桂宁[1,2] 吕纪华[1,2] 王丽[1,2] 刘瑛[1,2] 农志欢[1] HE Fei SU Hua WEI Gui-ning LV Ji-hua WANG Li LIU Ying NONG Zhi-huan(Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, Guangxi 530022, China Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standard, Nanning, Guangxi 530022, China)

机构地区：[1]广西中医药研究院,广西南宁530022 [2]广西中药质量标准研究重点实验室,广西南宁530022

出　　处：《中国热带医学》2016年第9期880-882,共3页China Tropical Medicine

基　　金：广西科学研究与技术开发计划项目(No.桂科攻13349007)

摘　　要：目的以MCHC、MCH和Hb A、Hb A2、Hb F等血象为观察指标,探讨乙基亚硝基脲(ethylnitrosourea,ENU)复制β-地贫模型的可行性。方法实验设模型组和正常对照组两组。模型组:先给雄性DBA/2J小鼠腹腔注射乙基亚硝基脲(200 mg/kg体重),经9周诱发遗传基因突变,然后将其与正常雌性C57BL/6J小鼠杂交繁育出具有β-地中海贫血特征的模型杂交F1代小鼠。正常对照组:将正常雄性DBA/2J小鼠与正常雌性C57BL/6J小鼠杂交繁育出正常杂交F1代小鼠。结果与正常杂交F1代小鼠比较,模型杂交F1代的部分小鼠血MCHC、MCH、Hb A显著降低,Hb A2和Hb F显著升高(P<0.05或P<0.01)与临床初步诊断患者为β-地中海贫血的相应血象指标的变化趋势相一致。结论乙基亚硝基脲可诱发小鼠类似β-地中海贫血。Objective To investigate the feasibility of ethylnitrosourea（ENU）in the establishment of mouse model of β-thalassemia using blood markers MCHC,MCH,Hb A,Hb A2 and Hb F as observational indicators. Methods DBA/2J micewere randomly divided into model group and normal control group. In the model group,model hybrid F1 mice were bred by ENU（200 mg/kg,for 9 weeks）-induced male DBA/2J mice and normal female C57BL/6J mice. In the normal group,the normalhybrid F1 mice were bred by normal male DBA/2J mice and normal female C57BL/6J mice. Results Compared with thenormal hybrid F1 mice,some mice from the model hybrid F1 mice showed significantly decreased MCHC,MCH and Hb A,andobviously increased Hb A2 and Hb F（P0.05 or P0.01）,which were consistence with the changes in the freshly diagnosed β-thalassemia patients. Conclusion ENU is liable to induce β-thalassemia-like condition in mice.

关 键 词：乙基亚硝基脲 Β-地中海贫血 动物模型 小鼠 

分 类 号：R556.61[医药卫生—血液循环系统疾病]