头穴丛刺对阿尔茨海默病大鼠MAPK信号的影响  

Cluster needling of scalp point therapyon on MAPK signaling pathway of Alzheimer's disease rats

作　　者：李虹霖[1] 徐飞[2] 夏昆鹏[1] 张淼[1] 王玉珏[1] 

机构地区：[1]黑龙江中医药大学附属二院,哈尔滨150001 [2]黑龙江省医院,哈尔滨150036

出　　处：《吉林中医药》2016年第9期925-928,共4页Jilin Journal of Chinese Medicine

基　　金：黑龙江省自然科学基金(H201476);黑龙江省博士后流动站资助基金(LBH-Z14199)

摘　　要：目的观察头穴丛刺对阿尔茨海默病(AD)大鼠MAPK信号中的erk、jnk、p38表达的影响。方法大鼠双侧海马区注射Aβ1-42建立AD大鼠模型,将60只大鼠随机分为5组:空白组,模型组,头穴丛刺组,传统头针组及体针组,每组各12只。经过4周干预后,经免疫组化法观察大鼠海马区组织中p-jnk/jnk、p-erk/erk、p-p38/p38的变化。结果头穴丛刺组、传统头针组分别与模型组相比差异具有统计学意义(P<0.05),体针组与模型组相比差异无统计学意义(P>0.05);p-jnk/jnk表达变化:各治疗组间相比差异均具有统计学意义(P<0.05),头穴丛刺组优于传统头针组及体针组;p-erk/erk表达变化:体针组与头穴丛刺组和传统头针组相比差异具有统计学意义(P<0.05),头穴丛刺组较优,头穴丛刺组与传统头针组相比有上升趋势,但P>0.05,两者比较差异无统计学意义;p-p38/p38表达变化:各治疗组间相比差异均具有统计学意义(P<0.05),头穴丛刺组优于传统头针组及体针组。结论头穴丛刺通过上调p-erk、下调p-jnk及p-p38的表达,进而改善大鼠学习记忆能力。Objective To explore the effect of cluster needling of scalp point therapy on erk,jnk and p38 of MAPK signaling pathway of alzheimer＇s disease rats. Methods Establishing model with Alzheimer＇s disease by injecting Aβ1-42 oligomer into bilateral hippocampus. Sixty rats were randomized into five groups including blank control group,cluster needling of scalp point group,traditional head acupuncture group,body acupuncture group,and model group,which contains twelve rats in each group. To observe the changes of p-jnk / jnk,p-erk / erk and p-p38 / p38 through immunohistochemical staining. Results It showed marked differences between each treatment group,excepting body acupuncture group,and the model group（ P ﹤ 0. 05）. While,there was no marked difference between the body acupuncture group and the model group（ P ﹥ 0. 05）. To observe the p-jnk / jnk： there was a statistical difference between each treatment group（ P ﹤ 0. 05）,and the cluster needling of scalp point group showed a better effect. To observe the p-erk / erk： it showed marked differences between body acupuncture group and the cluster needling of scalp point group,as well as the traditional head acupuncture group（ P ﹤ 0. 05）,and the cluster needling of scalp point group showed a better effect; it showed a rising trend comparing cluster needling of scalp point group and traditional head acupuncture group,but no marked differences between two groups（ P ﹥ 0. 05）. To observe the p-p38 / p38：tthere was a statistical difference between each treatment group（ P ﹤ 0. 05）,and the cluster needling of scalp point group showed a better effect. Conclusion The cluster needling of scalp point therapy can improve learning and memory function of Alzheimer＇s disease rats through up-regulating the p-erk / erk and down-regulating the p-jnk / jnk and pp38 / p38.

关 键 词：头穴丛刺 p-jnk/jnk p-erk/erk p-p38/p38 

分 类 号：R745.1[医药卫生—神经病学与精神病学]