caveolin-1和P21在罗红霉素抑制哮喘离体气道平滑肌细胞增殖中的作用  

The role of caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro

作　　者：王瑞丽[1] 戴威[1] 戴元荣[2] 

机构地区：[1]温州医科大学附属第二医院重症医学科,浙江温州325027 [2]温州医科大学附属第二医院呼吸内科,浙江温州325027

出　　处：《温州医科大学学报》2016年第9期629-632,共4页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(LY16H010007)

摘　　要：目的:探讨caveolae/caveolin-1和P21在罗红霉素抑制离体哮喘大鼠气道平滑肌细胞(ASMCs)增殖中的作用。方法:复苏冻存的哮喘ASMCs,CCK-8法检测不同浓度罗红霉素[A组(无罗红霉素)、R10组(罗红霉素10μg/mL)、R25组(罗红霉素25μg/mL)、R50组(罗红霉素50μg/mL)、R100组(罗红霉素100μg/mL)]干预哮喘ASMCs后细胞的增殖情况,透射电镜观察各组caveolae的表达,Western Blot法检测各组caveolin-1、P21的蛋白表达。结果:罗红霉素抑制哮喘ASMCs的增殖作用具有浓度依赖性,随罗红霉素浓度增加,caveolae的表达逐渐增加,caveolin-1、P21的蛋白表达亦逐渐增加,且R50组或R100组与A组、R10组相比差异有统计学意义(P<0.05)。相关分析示哮喘ASMCs增殖活性与caveolin-1、P21蛋白表达呈负相关(r=-0.881、r=-0.951,P<0.05),caveolin-1蛋白表达与P21蛋白表达呈正相关(r=0.957,P<0.01)。结论:罗红霉素可能通过上调caveolae/caveolin-1的表达,引起P21蛋白表达增加,从而抑制离体哮喘ASMCs的增殖。Objective： To explore the role of caveolae/caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro. Methods： Recovery freeze-stored ASMCs of asthmatic rats were divided into five groups [group A （blank group）, group R10 （roxithromycin 10 μg/mL）, group R25 （roxithromycin 25 μg/mL）, group R50 （roxithromycin 50 μg/mL） and group R100 （roxithromycin 100 μg/mL）]. The proliferation of ASMCs induced by roxithromycin were tested by CCK-8. The changes of caveolae in ASMCs were observed by transmission electron microscope. The protein expressions of caveolin-1 and P21 were measured by Western blot. Results： Roxithromycin could inhibit the proliferation of asthmatic rats ASMCs in vitro and had concentration-dependent manner. With the increased of intervention concentration, the expression of caveolae increased and the protein expressions of caveolin-1, P21 increased. Besides, compared with group A or group R10, the expressions of caveolin-1 and P21 protein in group R50 or R100 showed significantly different （P〈0.05）. There was significantly negative correlation between the cell activity rate of asthmatic rats and the expressions of caveolin-1 and P21 protein （r=-0.881, r=-0.951, P〈0.05）. The expression caveolin-1 was positively correlated with P21 （r=0.957, P〈0.01）. Conclusion： Roxithromycin inhibits the proliferation of ASMCs from asthmatic rats in vitro may partly through promoting the expression of P21 protein which induced by increased the expression of caveolae/ caveolin-1.

关 键 词：哮喘 气道平滑肌细胞 CAVEOLAE CAVEOLIN-1 P21 罗红霉素 

分 类 号：R56[医药卫生—呼吸系统]