B7-1、B7-2在牙龈卟啉单胞菌脂多糖诱导的T淋巴细胞反应中的作用  

Effect of B7-1 and B7-2 on T lymphocyte response induced by lipopolysaccharide of Porphyromonas gingivalis

作　　者：王莹 许庆安[1] 樊明文[1] Wang Ying Xu Qing-an Fan Ming-wen.(The State Key Laboratory Breeding Base of Basic Science of Stomatology （Hubei- MOST） ＆ Key Laboratory of Oral Biomedicine of Ministry of Education （ KLOBM） , School ＆ Hospital of Stomatology, Wuhan University, Hubei Wuhan 430079, China)

机构地区：[1]口腔基础医学省部共建国家重点实验室培育基地.口腔生物医学教育部重点实验室.武汉大学口腔医学院,湖北武汉430079

出　　处：《临床口腔医学杂志》2016年第9期515-519,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81271129)

摘　　要：目的:研究B7-1、B7-2分子对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)脂多糖诱导的T淋巴细胞反应的影响。方法:分别从基因敲除小鼠(B7-1 KO组、B7-2 KO组、B7-1/2 KO组)和野生小鼠(WT组)骨髓培养获得原代树突状细胞(dendritic cell,DCs)并经P.g脂多糖刺激后,与T细胞混合培养。CCK8试剂盒评价T细胞增殖,ELISA检测培养液中IFN-γ、IL-4及IL-17的水平,荧光定量PCR检测T细胞IFN-γ、IL-4、IL-17、GATA-3、T-bet及RORγt的转录水平。结果:CCK8检测显示各组T细胞增殖水平无差异。ELISA及荧光定量PCR显示与WT组比较,B7-1 KO组IFN-γ降低,IL-4升高,IL-17无变化,T-bet降低,GATA-3升高,RORγt无变化;B7-2 KO组IFN-γ升高,IL-4降低,IL-17降低,T-bet升高,GATA-3降低,RORγt降低;B7-1/2 KO组各因子变化趋势介于B7-1 KO组和B7-2 KO组之间。结论:B7-1及B7-2分子对P.g脂多糖刺激的T细胞增殖无明显影响。但B7-1分子可促进T细胞向Th1型分化,抑制Th2型分化,与Th17型分化相关性不大;而B7-2分子抑制T细胞向Th1型分化,促进Th2型及Th17型分化。Objective： To investigate the effect of B7-1 and B7-2 on T lymphocyte response induced by lipopolysaccharide（ LPS） of Porphyromonas gingivalis（ P. g）. Methods： Dendritic cells（ DCs） were cultured from the bone marrow of B7-1 KO,B7-2 KO,B7-1 /2 KO and wild type（ WT） mice. DCs were stimulated with LPS of P. g. in advance and then co-cultured with T lymphocytes. CCK8 kit was used to examine the proliferation of T lymphocytes. Cytokines such as IFN-γ,IL-4and IL-17 in the supernatants were detected by ELISA. Quantitative real-time PCR（ q PCR） was performed to test the RNA levels of IFN-γ,IL-4,IL-17,GATA-3,T-bet and RORγt of T cells. Results： There was no significant difference in T cell proliferation among groups. Compared with WT group,B7-1 KO group showed decreased IFN-γ and T-bet,increased IL-4 and GATA-3,and similar levels of IL-17 and RORγt. Whereas,B7-2 KO group showed higher levels of IFN-γ and T-bet,and lower levels of IL-4,GATA-3,IL-17 and RORγt than WT group. The variation tendency of these factors in B7-1 /2 KO group was between those of B7-1 KO group and B7-2 KO group. Conclusion： Deficency of B7-1 and / or B7-2 in DCs does not influence DCs-induced T lymphocyte proliferation. B7-1 may promote T lymphocyte differentiation to Th1 cells,inhibit its differentiation to Th2 cells,While B7-2 may inhibit T lymphocyte differentiation to Th1 cells and promote its differentiation to Th2 and Th17 cells.

关 键 词：牙龈卟啉单胞菌 脂多糖 B7-1 B7-2 T淋巴细胞 

分 类 号：R780.2[医药卫生—口腔医学] Q813.1[医药卫生—临床医学]