自体NK细胞过继输注对Ⅲ-Ⅳ期NSCLC患者天然免疫与体力状态的影响  

Immune function and performance score of patients with stage Ⅲ-Ⅳ non-small cell lung cancer patients before and after the adoptive transfer of autologous NK cells

作　　者：顾霞[1] 王凯玲[1] 芮庄华[1] 龙海虎 陈国君[2] 任涛[1] 

机构地区：[1]同济大学附属东方医院呼吸内科,上海200123 [2]同济大学附属东方医院血管外科,上海200123

出　　处：《同济大学学报（医学版）》2016年第4期70-73,82,共5页Journal of Tongji University(Medical Science)

基　　金：上海市优秀学术带头人计划(16XD1403100);浦东新区重点学科(PWZx2014-10)

摘　　要：目的研究自体NK细胞过继输注对晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)患者天然免疫及体力状况(performance status,PS)评分的影响。方法应用流式细胞仪检测20例晚期NSCLC患者(肺癌组)和20例健康人(健康对照组)外周血NK(CD16^+CD56^+)细胞的表达水平;检测晚期NSCLC患者应用自体NK细胞过继输注后外周血NK(CD16^+CD56^+)细胞的表达水平。根据美国东部肿瘤协作组(Eastern Cooperative Oncology Group,ECOG)体力状况评分标准,比较肺癌组患者应用自体NK细胞过继输注前后PS评分的变化情况。结果外周血NK(CD16^+CD56^+)细胞的表达明显降低[(8.980±1.942)%],与健康对照组[(15.290±1.869)%]比较差异均有统计学意义(P<0.05)。肺癌组治疗前PS评分为(1.50±1.080),应用自体NK细胞过继输注后PS评分显著降低(1.00±0.816),外周血NK(CD16^+CD56^+)细胞的表达显著升高[(24.660±10.049)%],与治疗前比较,差异均有统计学意义(P<0.05)。结论自体NK细胞过继免疫治疗有助于提高晚期NSCLC患者的免疫功能状态及体力状况。Objective To investigate the immune function and performance score of patients with advanced non-small cell lung cancer （NSCLC） before and after adoptive transfer of autologous NK cells. Methods Twenty patients with stage 11-IV NSCLC （Lung cancer group） undergoing adaptive autologous NK cells and 20 healthy subjects （control group） were enrolled in the study. Peripheral blood NK cells （CD16 ＋ CD56 ＊）were detected with flow cytometry, and the Eastern Cooperative Oncology Group（ECOG） performance scores （PS） were evaluated before and after the adoptive NK therapy. Results Before adaptive therapy the peripheral blood NK（ CD1＋ CD56＋ ）levels in NSCLC patients were significantly lower than those in controls [ （8. 980 ± 1. 942） % vs（ 15. 290 ± 1. 869） % , P〈0.05]. The PS was decreased from（1.50±1.080） to（1. 00 ±0. 816） （P 〈0. 05） ; and the peripheral blood NK levels was increased to（24. 660 ± 10. 049）% J after adoptive therapy（ P 〈0.05 ）. Conclusion Autologous NK cells adoptive immunotherapy may improve the immune function and performance status in patients with advanced non-small cell lung cancer.

关 键 词：非小细胞肺癌肿瘤 自体细胞过继免疫治疗 流式细胞术 外周血 NK细胞 体力状况评分 

分 类 号：R734.2[医药卫生—肿瘤]