肺癌组织中nucleostemin基因表达的研究  

Nucleostemin mRNA expression in lung cancer and its clinical significance

作　　者：熊峰 李俊 

机构地区：[1]荆州市传染病(胸科)医院病理室,湖北荆州434000

出　　处：《临床肺科杂志》2016年第11期2086-2087,2091,共3页Journal of Clinical Pulmonary Medicine

摘　　要：目的检测肺癌肿瘤组织中Nucleostemin基因表达的改变,探讨相关的临床意义。方法选取我院2014年3月-2016年2月收治的肺癌患者92例,另取我院同期因良性肺疾病30例活检肺组织作为对照。Nucleostemin基因表达量使用荧光定量PCR检测。结果 Nucleostemin在肺癌组织中表达量分别为0.318±0.076,显著高于对照肺组织的0.142±0.043(P<0.01)。男女性别、不同年龄、肿瘤大小以及病理类型之间Nucleostemin表达量无显著性差异(P>0.05)。Ⅲ、Ⅳ临床分期患者Nucleostemin表达量为0.364±0.077,显著高于Ⅰ、Ⅱ临床分期患者的0.286±0.051(P<0.05)。肿瘤有淋巴结转移患者Nucleostemin表达量为0.346±0.075,显著高于无淋巴结转移患者的0.271±0.048(P<0.05)。肿瘤中、低分化患者Nucleostemin表达量为0.331±0.073,显著高于高分化患者的0.282±0.054(P<0.05)。结论 Nucleostemin基因在肺癌组织中高表达,Nucleostemin基因表达量与肺癌的临床分期、有无淋巴结转移以及肿瘤分化程度密切相关。Objective To detect Nucleostemin mRNA expression in lung cancer and to explore its clinical significance. Methods 92 patients of lung cancer were enrolled in this study. Another 30 cases of benign lung tis-sues were used as controls. Real-time PCR analysis was used to detect Nucleostemin mRNA expression. Results Nucleostemin mRNA expression in lung cancer tissue was 0. 318 ± 0. 076, which was higher than that of 0. 142 ± 0. 043 in control tissue （P〈0. 01）. Nucleostemin mRNA expression had no significant difference in gender, age, tumor sizes, and pathological type in lung cancer patients （P〉0. 05）. Nucleostemin mRNA expression in theⅢandⅣ stage group was 0. 364 ± 0. 077, which was higher than that of 0. 286 ± 0. 051 in theⅠandⅡstage group （P〈0. 05）. Nucleostemin mRNA expression in the lymph node metastasis group was 0. 346 ± 0. 075, which was higher than that of 0. 271 ± 0. 048 in the non-lymph node metastasis group （P〈0. 05）. Nucleostemin mRNA expression in the median and low differentiation group was 0. 331 ± 0. 073, which was higher than that of 0. 282 ± 0. 054 in the well differentiation group group （ P 〈0. 05 ） . Conclusion The high expression of Nucleostemin mRNA is detected in lung cancer. which is related to clinical stages, lymph node metastasis, and tumor differentiation of lung cancer.

关 键 词：肺癌 NUCLEOSTEMIN基因 

分 类 号：R734.2[医药卫生—肿瘤]