超高效液相色谱同时测定渔用饲料中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素  

Simultaneous determination of bisdemethoxycurcumin,demethoxycurcumin and curcumin in fishery feed by UPLC

作　　者：刘永涛[1,2] 李乐[3] 徐春娟[4] 杨移斌[1] 董靖[1,2] 胥宁[1,2] 杨秋红[1] 艾晓辉[1,2] LIU Yongtao LI Le XU Chunjuan YANG Yibin DONG Jing XU Ning YANG Qiuhong AI Xiaohui(Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China Hubei Freshwater Aquaculture Collaborative Innovation Center,Wuhan 430223, China Chinese Academy of Fishery Sciences, Beijing 100141, China Shanghai Ocean University, Shanghai 201306, China)

机构地区：[1]中国水产科学研究院长江水产研究所,湖北武汉430223 [2]淡水水产健康养殖湖北省协同创新中心,湖北武汉430223 [3]中国水产科学研究院,北京100141 [4]上海海洋大学,上海201306

出　　处：《中国渔业质量与标准》2016年第5期60-64,共5页Chinese Fishery Quality and Standards

基　　金：资助项目:典型化学物污染淡水水产品质量安全综合防治技术方案(鄂;皖;川;渝片区)(201503108-C-C-1);中国水产科学研究院基本科研业务费(2016JBF0104)

摘　　要：姜黄素是一种新的渔用饲料添加剂,但目前尚无测定鱼饲料中姜黄素类化合物添加量检测方法。本研究建立了鱼饲料中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素同时测定的超高效液相色谱紫外法（UPLC-TUV）。方法采用乙醇作第一提取剂,乙腈为第二提取剂,基质标准曲线定量。以乙腈-0.1%乙酸水溶液（45∶55,V/V）为流动相,C18柱（2.1 mm×100 mm,1.7μm）为色谱柱,430 nm波长下进行检测。鱼饲料中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素最低检测限为10μg/kg,定量限为25μg/kg。在空白鱼饲料中添加双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素的水平在0.10-2.0 mg/kg时,平均回收率为87.68%-102.06%,相对标准偏差为1.06%-8.01%。本方法为检测鱼饲料中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素的添加量提供了一种技术手段。Since there is no analytical method for determination of 3 curcuminoids in fishery feed, it is necessary to es- tablish an ultra - high performance liquid chromatographic （ UPLC ） method for simultaneous determination of bisde- methoxycurcumin, demethoxycurcumin and curcumin in fishery feed. Ethanol was used as the first extraction solvent, and acetonitrile as the second extraction solvent, and matrix - fortified standard curve for quantification. Analytes were separated on C18 column （2.1 mm×100 mm） and detected at 430 nm wavelength by UPLC. The detection lim- its and quantification limits for bisdemethoxycurcumin, demethoxycurcumin and curcumin were both 10 and 25 μg/kg in fishery feed. The average recoveries of bisdemethoxycurcumin, demethoxycurcumin and curcumin in fishery feed were from 87.68% to 102.06% at the spiked level of 0.10 - 2.0 mg/kg. The relative standard deviations were from 1.06% to 8.01%. The present method provides a technical means for simultaneous determination of bisdemethoxy- curcumin, demethoxycurcumin and curcumin in fishery feed.

关 键 词：渔用饲料 双去甲氧基姜黄素 去甲氧基姜黄素 姜黄素 超高效液相色谱 

分 类 号：S963[农业科学—水产养殖]