杜仲醇的制备及其表征  

Preparation and Characterization of Eucommiol

作　　者：郑阳[1] 彭胜[1] 史丽娟[1] 彭密军[1,2] ZHENG Yang PENG Sheng SHI Li-juan PENG Mi-jun(Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Hunan Engineering Laboratory for Eucommia ulmoides Oliv., Jishou University, Zhangjiajie 427000, China Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410000, China)

机构地区：[1]吉首大学林产化工工程湖南省重点实验室杜仲湖南省工程实验室,张家界427000 [2]湖南省植物功能成分利用协同创新中心,长沙410000

出　　处：《天然产物研究与开发》2016年第9期1434-1436,1421,共4页Natural Product Research and Development

基　　金：湖南省教育厅高校产业化培育项目(11CY015);吉首大学林产化工工程湖南省重点实验室开放项目(JDZ201501);吉首大学校级科研项目(14JDY056)

摘　　要：采用大孔吸附树脂-制备液相色谱联用技术分离制备杜仲醇。以杜仲叶为原料,采用超声波辅助提取,提取液经过正丁醇萃取、D101大孔吸附树脂柱分离纯化后,再通过反相半制备液相色谱分离,以V(甲醇):V(水)=15∶85为流动相进行洗脱,制备得到杜仲醇单体。采用紫外光谱(UV)、红外光谱(IR)、核磁共振波谱(NMR)及液相色谱-质谱联用(LC-MS)等方法对所得单体进行了结构验证。液相色谱法分析检测表明制备所得杜仲醇纯度达95.12%。In this study,eucommiol was isolated and purified from leaves of Eucommia ulmoides Oliv. by liquid-liquid extraction,macroporous resin adsorption and semi-preparative HPLC. Firstly,the crude extract was extracted by ultrasonicassisted extraction using n-butanol as extraction solvent. Secondly,the n-butanol extract was separated by D101 macroporous resin column. Finally,eucommiol was prepared by reverse phase semi-preparative HPLC. The mobile phase of semi-preparative HPLC was methanol-water（ V∶ V = 15 ∶ 85）. The structure of the isolated eucommiol was subsequently confirmed by ultraviolet spectrophotometry（ UV）,infrared absorption spectroscopy（ IR）,nuclear magnetic resonance spectroscopy（ NMR） and liquid chromatography-mass spectrometry（ LC-MS）. HPLC analyses were performed to test and verify the purity of eucommiol（ 95. 12%）.

关 键 词：杜仲叶 杜仲醇 制备 结构表征 

分 类 号：R284.1[医药卫生—中药学]