Skp2与p27^(kip1)在人子宫颈鳞癌中的表达及其与HPV感染的关系  

Expression of Skp2 and p27^(kip1) in human cervical squamous cell carcinoma and its relationship with HPV infection

作　　者：刘求梅[1] 刘洁[1] 刘映 

机构地区：[1]湖南中医药高等专科学校基础医学部,湖南株洲412000 [2]株洲市中心医院病理科,湖南株洲412000

出　　处：《实用肿瘤杂志》2016年第5期429-433,共5页Journal of Practical Oncology

基　　金：湖南省卫生厅科研基金(2010-099)

摘　　要：目的 S期激酶相关蛋白2(S-phase kinase-associated protein 2,Skp2)与p27kip1在人子宫颈鳞癌中的表达及其与人乳头瘤病毒(human papilloma virus,HPV)感染的关系。方法选取子宫颈鳞状细胞癌(squamous cell carcinoma of cervix,SCC)组织50例、子宫颈上皮内瘤样病变(cervical intraepithelial neoplasias,CINs)组织40例,并选取慢性子宫颈炎(chronic cervicitis,CC)组织15例为对照组。用免疫组织化学法检测p27kip1和Skp2的表达情况,并与临床病理特征进行比较;用原位杂交法检测HPV 16/18的表达,及其与Skp2和p27kip1表达的相关性。结果 50例SCC组织中Skp2蛋白表达阳性率为76.0%,高于CC(13.3%,P<0.01)及CINs(57.5%,P<0.05)组织;p27kip1蛋白在SCC组织中表达阳性率为24.0%,低于CC(92.6%,P<0.01)及CINs(52.5%,P<0.05)组。Skp2和p27kip1的表达在淋巴结转移方面差异有统计学意义(P<0.05),而在年龄、分化程度、临床分期方面差异均无统计学意义(均P>0.05)。子宫颈鳞癌组织中HPV 16/18 DNA表达阳性率为74.0%,高于CC组的13.3%,组间差异有统计学意义(P<0.01);但与CINs组表达阳性率(62.5%)比较差异无统计学意义(P>0.05)。HPV 16/18感染与Skp2蛋白表达之间呈正相关(r=0.238,P<0.05),HPV 16/18感染与p27kip1蛋白表达之间呈负相关(r=-0.245,P<0.05)。结论 Skp2的高表达和p27kip1蛋白低表达与子宫颈鳞癌的发生、转移以及与HPV感染有密切关系。Objective To study the expression of S-phase kinase-associated protein 2（ Skp2） and p27^kip1 in human cervical squamous cell carcinoma and its relationship with human papilloma virus（ HPV） infection. Methods The expressions of Skp2 and p27^kip1 were detected by immunohistochemistry in 50 cases of squamous cell carcinoma of cervix（ SCC）,40 cases of cervical intraepithelial neoplasias（ CINs） and 15 cases of chronic cervicitis（ CC）. The expression of HPV16 /18 was detected by in situ hybridization. The association of Skp2 and p27^kip1 expression with clinicopathological features and with HPV16 /18 expression in cervical carcinoma was analyzed. Results The positive rate of Skp2 protein expression in SCC was 76. 0%,which was significantly higher than those in CC（ 13. 3%,P〈0. 01） and CINs（ 57. 5%,P〈0. 05）. The positive rate of p27^kip1 expression in SCC group was 24. 0%,which was significantly lower than those in CC（ 92. 6%,P〈0. 01） and CINs（ 52. 5%,P〈0. 05）. The expression levels of Skp2 and p27^kip1 were significantly associated with lymph node metastasis（ P〈0. 05）,but not with age,differentiation degree or clinical stage（ P〈0. 05）. The positive rate of HPV16 /18 DNA in SCC was 74. 0%,which was higher than that in CC（ 13. 3%,P〈0. 01）,but was not significantly different to that in CINs（ 62. 5%,P〈0. 05）. HPV16 /18 infection was positively correlated with the expression of Skp2（ r = 0. 238,P〈0. 05）,and negatively correlated with the level of p27^kip1（ r =- 0. 245,P〈0. 05） in cervical carcinoma. Conclusion High expression of Skp2 and low expression of p27^kip1 are closely related to lymph node metastasis of cervical squamous cell carcinoma and HPV infection.

关 键 词：宫颈肿瘤/病理学 宫颈肿瘤/病毒学 癌 鳞状细胞/病理学 癌 鳞状细胞/病毒学 S期激酶相关蛋白质类/生物合成 周期素依赖激酶抑制剂p27/生物合成 乳头状瘤病毒感染 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤] R730.23[医药卫生—临床医学]