HIWI在子宫内膜癌组织中的表达及临床意义  

Expression of HIWI in endometrial carcinoma and its clinical significance

作　　者：高鑫[1] 李艳琴[1] 韩代花 梁裕杰[1] 高俊[1] 

机构地区：[1]双流县妇幼保健院妇产科,四川成都610200

出　　处：《实用肿瘤杂志》2016年第5期437-441,共5页Journal of Practical Oncology

摘　　要：目的探讨人HIWI基因在子宫内膜癌组织中的表达及其临床意义。方法采用实时荧光定量PCR法（quantitative real-time reverse transcription-polymerase chain reaction,QRT-PCR）检测HIWI在子宫内膜癌细胞株（HEC-1B,RL95-2）及66例子宫内膜癌组织中的表达,分析其表达与患者临床病理特征及预后的关系。结果 HIWI mRNA在子宫内膜癌细胞株（HEC-1B,RL95-2）及癌组织标本中的表达较正常子宫内膜细胞株（ESC,NSC）及组织增高（均P〈0.01）,HIWI的表达在肿瘤肌层浸润深度、子宫颈浸润、淋巴血管侵犯、淋巴结转移和临床分期方面差异均有统计学意义（均P〈0.01）,在患者的年龄及组织病理分化程度方面差异均无统计学意义（均P〉0.05）。高表达HIWI的患者生存时间较低表达者缩短（P〈0.01）。HIWI可作为预测子宫内膜癌无进展生存时间（HR=5.18,95%CI：2.380-12.41,P〈0.01）和总生存时间（HR=3.27,95%CI：1.68-5.46,P〈0.01）的独立预后因子。结论 HIWI参与调节子宫内膜癌的发生、发展,可作为子宫内膜癌诊断和预后评估的分子标志物。Objective To study the expression of HIWI in human endometrial carcinoma and its clinical significance. Methods Sixty-six patients with endometrial carcinoma were enrolled in the study. The expression of HIWI in endometrial cancer cells and cancer tissues was detected by quantitative real-time reverse transcription-polymerase chain reaction（ QRT-PCR）. The association of HIWI expression with clinicopathological features and prognosis was analyzed. Results The expression level of HIWI mRNA was significantly higher in endometrial cancer cells（ HEC-1B and RL95-2）and cancer tissues than that in normal endometrial cells（ ESC and NSC） and tissues（ P〈0. 01）. Besides,HIWI expression was significantly correlated with tumor myometrial invasion depth,cervical invasion,lymph vascular invasion,lymph node metastasis and clinical stage（ P〈0. 01）,but was not correlated with age of patients or pathological differentiation of tumors（ P〈0. 05）. Patients with high HIWI expression had shorter survival time（ P〈0. 01）. HIWI was an independent prognostic factor for progression free survival time（ HR = 5. 18,95% CI： 2. 380-12. 41,P〈0. 01） and overall survival time（ HR = 3. 27,95% CI： 1. 68-5. 46,P〈0. 01）. Conclusion HIWI may be involved in the progression of endometrial carcinoma,and is a potential molecular marker for the pathological diagnosis and prognosis evaluation of endometrial carcinoma.

关 键 词：子宫内膜肿瘤/病理学 基因表达 蛋白质类/遗传学 聚合酶链反应 预后 

分 类 号：R737.33[医药卫生—肿瘤] R730.23[医药卫生—临床医学]