QuEChERS-气相色谱-质谱联用法测定育发类化妆品中的斑蝥素  

Determination of cantharidin in hair care cosmetics by QuEChERS-gas chromatography-mass spectrometry

作　　者：刘运明[1] 姜新[1] LIU Yun-Ming JIANG Xin(Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China)

机构地区：[1]江苏省疾病预防控制中心,南京210009

出　　处：《江苏预防医学》2016年第5期534-536,共3页Jiangsu Journal of Preventive Medicine

摘　　要：目的 建立QuEChER孓气相色谱质谱法测定育发类化妆品中斑蝥素的分析方法。方法用丙酮溶解样品，超声、离心后取上清液，加人分散固相萃取吸附剂（PSA粉末、GCB粉末和无水硫酸镁）净化，冷冻离心后取上清液浓缩，供气相色谱-质谱分析。结果斑蝥索在浓度0．1～2．5／μg／mL范围线性良好（r2=0．999 5），在0．1～2．5 μg／g添加水平回收率为86．0％～92．8％，相对标准偏差（RSD）为5．2％～7．3％；检出限（LOD）为0．02／μg／g，定量限（LGQ）为0．08／μg／g。结论该方法能有效去除育发类化妆品中的复杂基质，灵敏度高，定性、定量准确，可满足测定基质复杂的育发类化妆品中斑蝥素检测的需要。Objective To develop a gas chromatography-mass spectrometric method for analysis of cantharidin in hair carecosmetics. Method Cosmetics samples were dissolved in acetone,follwed by ultrasonic treatment and centrifugation, the su-permatant was loaded to QuEChERS（PSA powders,GCB powders and Magnesium sulfate anhydrous）for purification, followedby frozen centrifugation and GC-MS analysis. Results Linear relationship in the range of 0.1-2.5/μg/mL were satisfactory（r2=0. 9995）. The recovery rates were in the range of 86.0%-92.8% with spiking samples of 0.1-2.5/μg/g. The relative stand-ard deviations were in the range of 5.2%-7.3% ,the limit of detection was 0.02/μg/g ,and the limit of quantification was 0.08/μg/g. Conclusion The established method can remove complex marries of cosmetic effectively, with high sensitivity and accu-racy of quantification and qualification, which can satisfy the requirements of cantharidin analysis in the presence of complexmatrices of hair care cosmetics.

关 键 词：QUECHERS 气相色谱-质谱 斑蝥素 育发类化妆品 

分 类 号：R113[医药卫生—公共卫生与预防医学]