纳米雄黄对卵巢癌细胞COC1凋亡的影响  

Apoptosis induced by nanoparticles in ovarian cancer COC1

作　　者：马淑云[1] 高尚风[2] 吴胜军[1] 张少华[1] 陈蕊[1] 王莉[3] 徐锐[3] 

机构地区：[1]西安医学院第一附属医院妇科,西安710077 [2]西安交通大学医学院第一附属医院妇产科,西安710061 [3]西安医学院第一附属医院科研科,西安710077

出　　处：《重庆医学》2016年第29期4041-4043,共3页Chongqing medicine

基　　金：陕西省自然科学基金资助项目(2012JM4051)

摘　　要：目的探讨纳米雄黄对卵巢癌细胞COC1的凋亡作用。方法 0.6μmol/L浓度的纳米雄黄作用于COC1细胞,于不同时间收集的细胞。倒置显微镜下观察细胞形态,流式细胞仪检测细胞凋亡率,RT-PCR检测细胞中Bax、Bcl-2和Caspase-3的mRNA的表达水平,蛋白免疫印迹法(Western blot)检测细胞中Bax、Bcl-2和Caspase-3蛋白的表达水平。结果 0.6μmol/L浓度的纳米雄黄作用于COC1细胞48h后出现显著的凋亡形态改变;纳米雄黄显著的促进细胞凋亡,并随着纳米雄黄处理COC1细胞时间的延长凋亡率显著性增加(P<0.05);随着纳米雄黄处理间的延长COC1细胞中的Bax和Caspase-3的mRNA和蛋白表达水平显著增加(P<0.05),Bcl-2的mRNA和蛋白表达水平显著降低(P<0.05)。结论纳米雄黄对COC1细胞有显著的促凋亡作用,其作用机制可能与升高Bax和Caspase-3的表达和降低Bcl-2的表达有关。Objective To explore the apoptosis induced by nanoparticles in ovarian cancer COC1. Methods 0.6 μmol/L con- centration treated cells COCl,cells were collected at different times. Cell morphology was observed under an inverted microscope, rate of apoptosis were detected by flow cytometry. RT-PCR to detect the mRNA expression of Bax, Bcl-2 and Caspase-3 in the COC1 cells. Western blot to detect the protein expression of Bax,Bcl-2 and Caspase-3. Results Significant apoptotic morphological changes nanoparticles 0.6 μmol/L concentration appear to COC1 cells after 48 h; nanoparticles significantly promoted apoptosis and prolonged treatment with nanoparticles COC1 cell apoptosis rate significantly increased time （P〈0.05）; with the extension of nan- oparticles COC1 cell treatments in the Bax and mRNA and protein expression levels of Caspase-3 was significantly increased （P〈 0.05） ,mRNA and protein expression levels of Bcl-2 was significantly decreased （P〈0.05）. Conclusion Nanoparticles on COC1 cells have significant pro-apoptotic effect. The mechanism of action may be related to increased expression of Bax and Caspase-3, decreased expression of Bcl-2.

关 键 词：纳米雄黄 COC1细胞 BaxlBcl-2 CASPASE-3 

分 类 号：R737.31[医药卫生—肿瘤]