离子阱液质联用仪应用于植物叶片游离氨基酸快速分析方法的建立  

Simultaneous Analysis of 23 Amino Acids in Different Plant Tissues by Liquid Chromatography Ion Trap Tandem Mass Spectrometry

作　　者：张战凤[1] 李红霞[2] 樊永亮[1] 宋月[1] 武丽娟[1] 田宏刚[1] 刘小凤[1] 刘同先[1] 

机构地区：[1]西北农林科技大学植物保护学院,农业部西北黄土高原作物有害生物综合治理重点实验室,旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农业学报》2016年第8期1229-1236,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西北农林科技大学大型仪器设备新功能开发项目(dysb130103);国家重点基础研究发展计划(2013CB127600);陕西省农业科技创新与攻关项目(2015NY109)

摘　　要：建立一种液相色谱-离子阱串联质谱(LC-MS-MS)同时分析植物组织中23种游离氨基酸的方法。植物样品经盐酸-乙醇法一步提取后直接进样,无需进行衍生和固相萃取等其他前处理步骤。液相色谱采用Intertsil OSD-4C18(250mm×3.0mm)色谱柱,采用甲醇-甲酸体系进行洗脱,对23种氨基酸获得比较理想的分离效果。结果表明,该方法通用性良好、样品制备简单(一步提取,无需衍生)、灵敏度高、检出范围宽、高效快速,可以在30min内完成整个分析过程。A method for simultaneous analysis of 23 free amino acids in different plant tissues was developed using liquid chromatography ion trap tandem mass spectrometry under positive ionization mode. Plant samples were extracted directly using hydrochloric acid-ethanol solution, and analyzed without derivatization or solid phase extraction. The LC separation was carried on a reversed-phase Intertsil OSD-4 C18（250 mm ×3.0 mm ）with the mobile phase of methyl alcohol/formic acid/water solution in gradient mode. The results showed that the developed method is of good efficiency, sensitivity and selectivity, and has been successfully applied to determine the free amino acids in different plant tissues.

关 键 词：氨基酸 液相色谱-质谱联用 植物组织 

分 类 号：Q946[生物学—植物学] O657.63[理学—分析化学]