Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets  

低蛋白日粮对断奶仔猪胃肠道消化酶表达及激素分泌的影响（英文）

作　　者：Zhi-mei TIAN Xian-yong MA Xue-fen YANG Qiu-li FAN Yun-xia XIONG Yue-qin QIU Li WANG Xiao-lu WEN Zong-yong JIANG 

机构地区：[1]State Key Laboratory of Livestock and Poultry Breeding,Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China,Guangdong Public Laboratory of Animal Breeding and Nutrition,Guangdong Key Laboratory of Animal Breeding and Nutrition,Institute of Animal Science,Guangdong Academy of Agricultural Sciences

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2016年第10期742-751,共10页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Basic Research Program(973)of China(Nos.2013CB127301 and 2013CB127304);the Guangdong International Science and Technology Cooperation Program(No.2014A050503049);the China Agriculture Research System(No.CARS-36);the Science and Technology Program of Guangdong Province(Nos.2013A061401020 and 2016A020210041),China

摘　　要：To investigate dietary protein level effects on digestive mechanisms, weaned piglets were fed for 45 d with diets containing 20%, 17%, or 14% crude protein （CP） supplemented to meet requirements for essential amino acids. This article describes the influence of dietary protein on gastrointestinal hormones and expression of an array of digestive enzymes in the gastrointestinal tract and pancreas. Results indicated that there were no significant differ- ences in expression of enzymes involved in carbohydrate digestion, except for maltase in the duodenum. In the je- junum, amylase expression in pigs fed 20% CP was much higher than that in pigs fed other diets （P〈0.05） and maltase expression in those fed 17% CP was higher than that in other treatments （P〈0.05）. Although there were no remarkable differences in expression of aminopeptidase in the small intestine or carboxypeptidase in the pancreas （P〉0.05）, there was a trend towards higher expression of various proteases in pigs fed 17% CP. The duodenal expression of en- teropeptidase in diets with 14% and 17% CP was significantly higher than that with 20% CP （P〈0.05）, but treatment differences did not existed in jejunum （P〉0.05）. The expression of GPR93 as a nutrient-responsive G protein-coupled receptor in 14% and 17% CP diets was significantly higher than that in 20% CP diet in the small intestine （P〈0.05）. The expressions of genes for pancreatic enzymes, lipase and elastase, were significantly higher in pigs fed diets with low CP, while similar trends occurred for carboxypeptidase, chymotrypsin and amylase. Conversely, the gastric expres- sions of pepsinogen A and progastricsin were lower with the 17% CP diet. Differences between treatments were found in the gastric antral contents of cholecystokinin and somatostatin： both increased in pigs fed 17% CP, accompanied by decreased content of motilin, which was also seen in plasma concentrations. These patterns were not reflected in duodenal contents. In general, 17% dietary CP 目的:研究蛋白日粮水平对断奶仔猪消化的影响机制。创新点:从营养物质消化及激素变化方面研究低蛋白日粮对仔猪胃肠道消化吸收的影响,探究低蛋白日粮饲养的可行性。方法:18头28日龄断奶仔猪经过7天适应期后平均体重为(9.57±0.64)kg。仔猪随机分为三组,每组6头,分别饲喂20%、17%和14%粗蛋白日粮并自由采食45天。根据国家研究委员会(NRC)2012年的标准,通过添加赖氨酸、蛋氨酸、半胱氨酸、苏氨酸及色氨酸等理想蛋白模型满足仔猪生长需求。实验结束后,进行颈静脉采血并屠宰取样。结论:结果表明基于蛋白日粮水平对内分泌及消化酶影响,与14%及20%粗蛋白组相比,17%蛋白日粮更有利于胃肠道和胰腺等组织中三种主要营养物质的消化。因此,17%粗蛋白水平更适合断奶仔猪的营养需求,证实了低蛋白日粮对断奶仔猪饲养的可行性。

关 键 词：Dietary protein PIGLET Gastrointestinal tract PANCREAS DIGESTION HORMONE 

分 类 号：S828.5[农业科学—畜牧学]